Probing lanthanide ions binding on tRNA(Phe) by H-1 NMR
文献类型：期刊论文
| 作者 | Tu HM ; Yang YS ; Meng JX ; Gong ML ; Wu YJ ; Zhang HJ |
| 刊名 | journal of rare earths
 |
| 出版日期 | 1998 |
| 卷号 | 16期号:3页码:161-166 |
| 关键词 | PHENYLALANINE TRANSFER-RNA YEAST TRANSFER RNAPHE NUCLEAR MAGNETIC-RESONANCE CRYSTAL-STRUCTURE SPECTROSCOPY ASSIGNMENT PHE |
| ISSN号 | 1002-0721 |
| 中文摘要 | the structure of phenylalanine transfer ribonucleic acid (trna(phe)) in solution was explored by h-1 nmr spectroscopy to evaluate the effect of lanthanide ion on the structural and conformational change. it was found that la3+ ions possess specific effects on the imino proton region of the h-1 nmr spectra for yeast trna(phe). the dependence of the imino proton spectra of yeast trna(phe) as a function of la3+ concentration was examined, and the results suggest that the tertiary base pair g(15). c-48, which is located in the terminal in the augmented dihydrouridine helix (d-helix), was markedly affected by la3+ (shifted to downfield by as much as 0.35). base pair u-8. a(14) in yeast trna(phe), which are stacked on g(15). c-48, was also affected by added la3+ when 1 similar to 2 mg2+ were also present. another imino proton that may be affected by la3+ in yeast trna(phe) is that of the tertiary base pair g(19). c-56. the assignment of this resonance in yeast trna(phe) is tentative since it is located in the region of highly overlapping resonances beween 12.6 and 12.2. this base pair helps to anchor the d-loop to the t psi c loop. the binding of la3+ caused conformational change of trna, which is responsible for shifts to upfield or downfield in h-1 nmr spectra. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000076252300001 |
| 公开日期 | 2010-11-04 |
| 源URL | [http://202.98.16.49/handle/322003/22697]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Tu HM,Yang YS,Meng JX,et al. Probing lanthanide ions binding on tRNA(Phe) by H-1 NMR[J]. journal of rare earths,1998,16(3):161-166. |
| APA | Tu HM,Yang YS,Meng JX,Gong ML,Wu YJ,&Zhang HJ.(1998).Probing lanthanide ions binding on tRNA(Phe) by H-1 NMR.journal of rare earths,16(3),161-166. |
| MLA | Tu HM,et al."Probing lanthanide ions binding on tRNA(Phe) by H-1 NMR".journal of rare earths 16.3(1998):161-166. |
入库方式： OAI收割
来源：长春应用化学研究所
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