Molecular characterization of beef liver catalase by scanning tunneling microscopy
文献类型：期刊论文
| 作者 | Zhang JD ; Chi QJ ; Zhang BL ; Dong SJ ; Wang EK |
| 刊名 | electroanalysis
 |
| 出版日期 | 1998 |
| 卷号 | 10期号:11页码:738-746 |
| ISSN号 | 1040-0397 |
| 关键词 | ORIENTED PYROLYTIC-GRAPHITE ATOMIC FORCE MICROSCOPY TUNNELLING MICROSCOPY CARBON ELECTRODES ELECTROCHEMICAL ACTIVATION PROBE MICROSCOPY DNA SURFACES ENVIRONMENT OXIDATION |
| 通讯作者 | dong sj |
| 中文摘要 | beef liver catalase molecules can stick tenaciously to the highly oriented pyrolytic graphite (hopg) surface which has been activated by electrochemical anodization. the immobilized sample is stable enough for high resolution scanning tunneling microscope (stm) imaging. when the anodized conditions are controlled properly, the hopg surface will be covered with a very thin oxide layer which can bind the protein molecules. individual molecules of native beef liver catalase are directly observed in detail by stm, which shows an oval-shape structure with a waist. the dimensions of one catalase molecule in this study are estimated as 9.0 x 6.0x 2.0 nm(3), which are in good agreement with the known data obtained from x-ray analysis, except the height can not be exactly determined from stm. electrochemical results confirm that the freshly adsorbed catalase molecules maintain their native structures with biological activities. however, the partly unfolding structure of catalase molecules is observed after the sample is stored for 15 days, this may be caused by the long-term interaction between catalase molecules and the anodized hopg surface. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000076181100002 |
| 公开日期 | 2010-11-04 |
| 源URL | [http://202.98.16.49/handle/322003/22707]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhang JD,Chi QJ,Zhang BL,et al. Molecular characterization of beef liver catalase by scanning tunneling microscopy[J]. electroanalysis,1998,10(11):738-746. |
| APA | Zhang JD,Chi QJ,Zhang BL,Dong SJ,&Wang EK.(1998).Molecular characterization of beef liver catalase by scanning tunneling microscopy.electroanalysis,10(11),738-746. |
| MLA | Zhang JD,et al."Molecular characterization of beef liver catalase by scanning tunneling microscopy".electroanalysis 10.11(1998):738-746. |
入库方式： OAI收割
来源：长春应用化学研究所
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