Viologen-thiol self-assembled monolayers for immobilized horseradish peroxidase at gold electrode surface
文献类型：期刊论文
| 作者 | Li JH ; Yan JC ; Deng Q ; Cheng GJ ; Dong SJ |
| 刊名 | electrochimica acta
 |
| 出版日期 | 1997 |
| 卷号 | 42期号:6页码:961-967 |
| 关键词 | CYTOCHROME-C GLUCOSE-OXIDASE DIRECT ELECTROCHEMISTRY NITRATE REDUCTASE REDOX CONVERSION AMINO-ACID ENZYME PROTEINS KINETICS SYSTEMS |
| ISSN号 | 0013-4686 |
| 中文摘要 | a stable, well-behaved self-assembled monolayer (sam) of viologen-functionalized thiol was used to immobilize and electrically connect horseradish peroxidase (hrp) at gold electrode. viologen groups in sams facilitated the electron transfer from the electrode to the protein active site so that hrp exhibited a quasi-reversible redox behavior. hrp adsorbed in the sams is very stable, and close to a monolayer with the surface coverage of 6.5 x 10(-11) mol/cm(2). the normal potential of hrp is -580 mv vs ag/agcl corresponding to ferri/ferro active center and the standard electron transfer rate constant is 3.41 s(-1) in 0.1 m phosphate buffer solution (ph 7.1). this approach shows a great promise for designing enzyme electrodes with other redox proteins and practical use in tailoring a variety of amperometric biosensor devices. copyright (c) 1997 elsevier science ltd. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| 公开日期 | 2010-12-22 ; 2011-06-09 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/24447]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li JH,Yan JC,Deng Q,et al. Viologen-thiol self-assembled monolayers for immobilized horseradish peroxidase at gold electrode surface[J]. electrochimica acta,1997,42(6):961-967. |
| APA | Li JH,Yan JC,Deng Q,Cheng GJ,&Dong SJ.(1997).Viologen-thiol self-assembled monolayers for immobilized horseradish peroxidase at gold electrode surface.electrochimica acta,42(6),961-967. |
| MLA | Li JH,et al."Viologen-thiol self-assembled monolayers for immobilized horseradish peroxidase at gold electrode surface".electrochimica acta 42.6(1997):961-967. |
入库方式： OAI收割
来源：长春应用化学研究所
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