In situ electrochemical scanning tunnelling microscopy investigation of structure for horseradish peroxidase and its electrocatalytic property
文献类型：期刊论文
| 作者 | Zhang JD ; Chi QJ ; Dong SJ ; Wang EK |
| 刊名 | bioelectrochemistry and bioenergetics
 |
| 出版日期 | 1996 |
| 卷号 | 39期号:2页码:267-274 |
| 关键词 | ORIENTED PYROLYTIC-GRAPHITE ATOMIC FORCE MICROSCOPY TUNNELING-MICROSCOPY MOLECULAR-STRUCTURE CARBON ELECTRODES DNA ACTIVATION SURFACE ACID MECHANISM |
| ISSN号 | 0302-4598 |
| 中文摘要 | immobilization of protein molecules is a fundamental problem for scanning tunnelling microscopy (stm) measurements with high resolution. in this paper, an electrochemical method has been proved to be an effective way to fix native horseradish peroxidase (hrp) as well as inactivated hrp from electrolyte onto a highly oriented pyrolytic graphite (hopg) surface. this preparation is suitable for both ex situ and in situ electrochemical stm (ecstm) measurements. in situ stm has been successfully employed to observe totally different structures of hrp in three typical cases: (1) in situ ecstm reveals an oval-shaped pattern for a single molecule in neutral buffer solution, which is in good agreement with the dimension determined as 6.2 x 4.3 x 1.2. nm(3) by ex situ stm for native hrp; (2) in situ ecstm shows that the adsorbed hrp molecules on hopg in a denatured environment exhibit swelling globes at the beginning and then change into a v-shaped pattern after 30 min; (3) in situ ecstm reveals a black hole in every ellipsoidal sphere for inactivated hrp in strong alkali solution. the cyclic voltammetry results indicate that the adsorbed native hrp can directly catalyse the reduction of hydrogen peroxide, demonstrating that a direct electron transfer reduction occurred between the enzyme and hopg electrode, whereas the corresponding cyclic voltammograms for denatured hrp and inactivated hrp adsorbed on hopg electrodes indicate a lack of ability to catalyse h2o2 reduction, which confirms that the hrp molecules lost their biological activity. obviously, electrochemical results powerfully support in situ stm observations. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| 公开日期 | 2010-12-22 ; 2011-06-09 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/25487]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhang JD,Chi QJ,Dong SJ,et al. In situ electrochemical scanning tunnelling microscopy investigation of structure for horseradish peroxidase and its electrocatalytic property[J]. bioelectrochemistry and bioenergetics,1996,39(2):267-274. |
| APA | Zhang JD,Chi QJ,Dong SJ,&Wang EK.(1996).In situ electrochemical scanning tunnelling microscopy investigation of structure for horseradish peroxidase and its electrocatalytic property.bioelectrochemistry and bioenergetics,39(2),267-274. |
| MLA | Zhang JD,et al."In situ electrochemical scanning tunnelling microscopy investigation of structure for horseradish peroxidase and its electrocatalytic property".bioelectrochemistry and bioenergetics 39.2(1996):267-274. |
入库方式： OAI收割
来源：长春应用化学研究所
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