The direct electrochemistry of cryo-hydrogel immobilized myoglobin at a glassy carbon electrode
文献类型：期刊论文
| 作者 | Niu JJ ; Guo YZ ; Dong SJ |
| 刊名 | journal of electroanalytical chemistry
 |
| 出版日期 | 1995 |
| 卷号 | 399期号:1-2页码:41-46 |
| 关键词 | HEART CYTOCHROME-C ALKALINE ISOMERIZATION GOLD ELECTRODE FILM ELECTRODE REDUCTION OXIDATION PROTEINS EQUILIBRIUM PH |
| ISSN号 | 0022-0728 |
| 中文摘要 | a cryo-hydrogel membrane (chm) immobilized at a glassy carbon (gc) electrode is reported for the direct electron transfer of redox proteins. the most attractive characteristics of this chm were its hydrophilic micro-environment for incorporated proteins to retain their activities, its high ability for protection against interference of denatured and adsorbed proteins at the electrode, its potential applications for various proteins or enzymes, as well as its high mechanical strength and thermal stability. a clear well developed and stable redox wave was obtained for commercially available horse heart myoglobin without further purification, giving a peak to peak separation delta e(p) = 93 mv at 5 mv s(-1) and the formal electrode potential e(0)' = -0.158 v (vs. ag/agcl). the formal heterogeneous electron transfer rate constant was calculated as k(0)' = 5.7 x 10(-4) cm s(-1) at ph 6.5, showing rapid electron transfer was achieved. the ph controlled conformational equilibria, acid state --> natural state --> basic i state --> basic ii state, of myoglobin at the chm gc electrode in the ph range 0-13.8 were also observed and are discussed in detail. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| 公开日期 | 2010-12-30 ; 2011-06-10 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/26353]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Niu JJ,Guo YZ,Dong SJ. The direct electrochemistry of cryo-hydrogel immobilized myoglobin at a glassy carbon electrode[J]. journal of electroanalytical chemistry,1995,399(1-2):41-46. |
| APA | Niu JJ,Guo YZ,&Dong SJ.(1995).The direct electrochemistry of cryo-hydrogel immobilized myoglobin at a glassy carbon electrode.journal of electroanalytical chemistry,399(1-2),41-46. |
| MLA | Niu JJ,et al."The direct electrochemistry of cryo-hydrogel immobilized myoglobin at a glassy carbon electrode".journal of electroanalytical chemistry 399.1-2(1995):41-46. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。