The conjugates of carbon nanodots and chlorin e6 for enhancing cellular internalization and photodynamic therapy of cancers
文献类型：期刊论文
| 作者 | Wang, Xiongwei; Liu, Jun; Huang, Zheng; Wang, Shimiao; Wang, Jing |
| 刊名 | Laser Phys. Lett.
 |
| 出版日期 | 2016 |
| 卷号 | 13期号:9 |
| 通讯作者 | jliu@siom.ac.cn |
| 英文摘要 | Chlorin e6 (Ce6), a large heterocyclic aromatic molecule, is a promising photosensitizer for photodynamic therapy (PDT). We propose an efficient nano-photosensitizer delivery system based on covalent interactions between Ce6 and polyethylenimine (PEI) coated carbon nanodots (CDots). We observed >50% Ce6 drug loading content for PEI, due to this compound's unique 'proton sponge effect.' We found that the covalently incorporated Ce6 molecules retained their functional properties for near-infrared (NIR) fluorescence imaging and PDT. The chemical characteristics of CDot-PEI-Ce6 and Ce6 were evaluated using different analytical methods, including transmission electron microscopy and UV-Visible absorption spectra. Time-correlated single photon counting (TCSPC) and fluorescence spectra were used to demonstrate that Ce6 successfully conjugated to the CDots. The high cellular uptake of CDots-PEI-Ce6 was confirmed using flow cytometry and confocal laser scanning microscopy. According to the MTT assay, the CDots-PEI-Ce6 exhibited low dark toxicity and efficient PDT efficacy to HeLa cancer cells. These results indicate that CDot-PEI-Ce6 conjugates are potential photosensitizer delivery systems for PDT. |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China (NSFC) [11274327, 61521093, 61527821]; Chinese Academy of Sciences [YZ201538] |
| WOS记录号 | WOS:000383980900011 |
| 源URL | [http://ir.siom.ac.cn/handle/181231/27743]  |
| 专题 | 上海光学精密机械研究所_强场激光物理国家重点实验室 |
| 作者单位 | 中国科学院上海光学精密机械研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Xiongwei,Liu, Jun,Huang, Zheng,et al. The conjugates of carbon nanodots and chlorin e6 for enhancing cellular internalization and photodynamic therapy of cancers[J]. Laser Phys. Lett.,2016,13(9). |
| APA | Wang, Xiongwei,Liu, Jun,Huang, Zheng,Wang, Shimiao,&Wang, Jing.(2016).The conjugates of carbon nanodots and chlorin e6 for enhancing cellular internalization and photodynamic therapy of cancers.Laser Phys. Lett.,13(9). |
| MLA | Wang, Xiongwei,et al."The conjugates of carbon nanodots and chlorin e6 for enhancing cellular internalization and photodynamic therapy of cancers".Laser Phys. Lett. 13.9(2016). |
入库方式： OAI收割
来源：上海光学精密机械研究所
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