Hypaphorine, an Indole Alkaloid Isolated from Caragana korshinskii Kom., Inhibites 3T3-L1 Adipocyte Differentiation and Improves Insulin Sensitivity in Vitro
文献类型：期刊论文
| 作者 | Luan, Guangxiang2,3; Tie, Fangfang2,3; Yuan, Zhenzhen2,3; Li, Gang2,4; He, Jie4; Wang, Zhenhua4; Wang, Honglun1,2 |
| 刊名 | CHEMISTRY & BIODIVERSITY
 |
| 出版日期 | 2017-07-01 |
| 卷号 | 14期号:7 |
| 关键词 | Isolation Hypaphorine Adipocyte differentiation Insulin resistance 3T3-L1 cells |
| 英文摘要 | Obesity, a major health problem worldwide, is a complex multifactorial chronic disease that increases the risk for insulin resistance, type 2 diabetes, coronary heart disease, and hypertension. In this study, we assessed methods to isolate hypaphorine, a potent drug candidate for obesity and insulin resistance. Semi-preparative reversed-phase liquid chromatography (semi-preparative RPLC) was established as a method to separate three compounds, adenosine, l-tryptophan, and hypaphorine, from the crude extracts of Caragana korshinskii Kom. Due to its specific chemical structure, the effect of hypaphorine on differentiation and dexamethasone (DXM) induced insulin resistance of 3T3-L1 cells was investigated. The structures of the three compounds were confirmed by UV, H-1-NMR, and C-13-NMR analysis and compared with published data. The activity results indicated that hypaphorine prevented the differentiation of 3T3-L1 preadipocytes into adipocytes by down-regulating hormone-stimulated protein expression of peroxisome proliferator activated receptor (PPAR) and CCAAT/enhancer binding protein (C/EBP), and their downstream targets, sterol regulatory element binding protein 1 c (SREBP1c) and fatty acid synthase (FAS). Hypaphorine also alleviated DXM-induced insulin resistance in differentiated 3T3-L1 adipocytes via increasing the phosphorylation level of Akt2, a key protein in the insulin signaling pathway. Taken together, we suggest that the method can be applied to large-scale extraction and large-quantity preparation of hypaphorine for treatment of obesity and insulin resistance. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | FUNGAL HYPAPHORINE ; ADIPOGENESIS ; PURIFICATION ; COMBINATION ; SEPARATION ; CELLS ; HSCCC ; CONSTITUENTS ; CHINENSIS ; RECEPTOR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000405127700009 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/7074]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining 810008, Peoples R China 2.Chinese Acad Sci, Key Lab Tibetan Med Res, Northwest Inst Plateau Biol, 23 Xinning Rd, Xining 810008, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Yantai Univ, Ctr Mitochondria & Healthy Aging, Coll Life Sci, 30 Qingquan Rd, Yantai 264005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luan, Guangxiang,Tie, Fangfang,Yuan, Zhenzhen,et al. Hypaphorine, an Indole Alkaloid Isolated from Caragana korshinskii Kom., Inhibites 3T3-L1 Adipocyte Differentiation and Improves Insulin Sensitivity in Vitro[J]. CHEMISTRY & BIODIVERSITY,2017,14(7). |
| APA | Luan, Guangxiang.,Tie, Fangfang.,Yuan, Zhenzhen.,Li, Gang.,He, Jie.,...&Wang, Honglun.(2017).Hypaphorine, an Indole Alkaloid Isolated from Caragana korshinskii Kom., Inhibites 3T3-L1 Adipocyte Differentiation and Improves Insulin Sensitivity in Vitro.CHEMISTRY & BIODIVERSITY,14(7). |
| MLA | Luan, Guangxiang,et al."Hypaphorine, an Indole Alkaloid Isolated from Caragana korshinskii Kom., Inhibites 3T3-L1 Adipocyte Differentiation and Improves Insulin Sensitivity in Vitro".CHEMISTRY & BIODIVERSITY 14.7(2017). |
入库方式： OAI收割
来源：西北高原生物研究所
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