A new combined method of stable isotope-labeling derivatization-ultrasound-assisted dispersive liquid-liquid microextraction for the determination of neurotransmitters in rat brain microdialysates by ultra high performance liquid chromatography tandem mass spectrometry
文献类型：期刊论文
| 作者 | Ji, Wenhua3; Geng, Yanling3; Wang, Xiao3; You, Jinmao1,2,4; Chen, Guang1,2; Zheng, Longfang1,2; Zhao, Xian-En1,2,3,4; Zhu, Shuyun1,2; Tao, Yanduo4 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2017-06-01 |
| 卷号 | 1054页码:64-72 |
| 关键词 | In vivo microdialysis Neurotransmitters Triple quadrupole mass spectrometry Sensitivity enhancement Stable isotope-labeling derivatization Targeted metabolomics |
| 英文摘要 | In this work, for the first time, a new hyphenated technique of stable isotope-labeling derivatization-ultrasound-assisted dispersive liquid-liquid microextraction has been developed for the simultaneous determination of monoamine neurotransmitters (MANTs) and their biosynthesis precursors and metabolites. The developed method was based on ultra high performance liquid chromatography tandem mass spectrometry detection using multiple-reaction monitoring mode. A pair of mass spectrometry sensitizing reagents, d(0)-10-methyl-acridone-2-sulfonyl chloride and d(3)-10-methyl-acridone-2-sulfonyl chloride, as stable isotope probes was utilized to facilely label neurotransmitters, respectively. The heavy labeled MANTs standards were prepared and used as internal standards for quantification to minimize the matrix effects in mass spectrometry analysis. Low toxic bromobenzene (extractant) and acetonitrile (dispersant) were utilized in microextraction procedure. Under the optimized conditions, good linearity was observed with the limits of detection (S/N > 3) and limits of quantification (S/N > 10) in the range of 0.002-0.010 and 0.015-0.040 nmol/L, respectively. Meanwhile, it also brought acceptable precision (4.2-8.8%, peak area RSDs %) and accuracy (recovery, 96.9-104.1%) results. This method was successfully applied to the simultaneous determination of monoamine neurotransmitters and their biosynthesis precursors and metabolites in rat brain microdialysates of Parkinson's disease and normal rats. This provided a new method for the neurotransmitters related studies in the future. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | SOLID-PHASE EXTRACTION ; MONOAMINE NEUROTRANSMITTERS ; UHPLC-MS/MS ; AMINO-ACID ; LC-MS/MS ; CATECHOLAMINE NEUROTRANSMITTERS ; CHEMILUMINESCENCE DETECTION ; SIMULTANEOUS QUANTIFICATION ; MULTIPLE NEUROTRANSMITTERS ; CAPILLARY-ELECTROPHORESIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000402219000009 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/7091]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Coll Chem & Chem Engn, Shandong Prov Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China 2.Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Shandong, Peoples R China 3.Shandong Acad Sci, Shandong Anal & Test Ctr, Key Lab TCM Qual Control Technol, 19 Keyuan St, Jinan 250014, Shandong, Peoples R China 4.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ji, Wenhua,Geng, Yanling,Wang, Xiao,et al. A new combined method of stable isotope-labeling derivatization-ultrasound-assisted dispersive liquid-liquid microextraction for the determination of neurotransmitters in rat brain microdialysates by ultra high performance liquid chromatography tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2017,1054:64-72. |
| APA | Ji, Wenhua.,Geng, Yanling.,Wang, Xiao.,You, Jinmao.,Chen, Guang.,...&Tao, Yanduo.(2017).A new combined method of stable isotope-labeling derivatization-ultrasound-assisted dispersive liquid-liquid microextraction for the determination of neurotransmitters in rat brain microdialysates by ultra high performance liquid chromatography tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1054,64-72. |
| MLA | Ji, Wenhua,et al."A new combined method of stable isotope-labeling derivatization-ultrasound-assisted dispersive liquid-liquid microextraction for the determination of neurotransmitters in rat brain microdialysates by ultra high performance liquid chromatography tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1054(2017):64-72. |
入库方式： OAI收割
来源：西北高原生物研究所
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