Anti-inflammatory bioactive equivalence of combinatorial components beta-carboline alkaloids identified in Arenaria kansuensis by two-dimensional chromatography and solid-phase extraction coupled with liquid-liquid extraction enrichment technology
文献类型：期刊论文
| 作者 | Mei, Lijuan1,3,4; Liu, Zenggen1,3,4 ; Cui, Yulei1,2,3,4; Shen, Na1,2,3,4; Dang, Jun1,3,4; Tao, Yanduo1,3,4
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| 刊名 | JOURNAL OF SEPARATION SCIENCE
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| 出版日期 | 2017-07-01 |
| 卷号 | 40期号:14页码:2895-2905 |
| 关键词 | Arenaria kansuensis bioactive equivalence of combinatorial components liquid-liquid extraction solid-phase extraction two-dimensional chromatography |
| 英文摘要 | Bioactive equivalent combinatorial components play a critical role in herbal medicines. However, how to discover and enrich them efficiently is a question for herbal pharmaceuticals researchers. In our work, a novel two-dimensional reversed-phase/hydrophilic interaction high-performance liquid chromatography method was established to perform real-time components trapping and combining for preparation and isolation of coeluting components. Arenaria kansuensis was taken as an example, and solid-phase extraction coupled with liquid-liquid extraction as a simple and efficient method for enriching trace components, reversed phase column coupled with hydrophilic interaction liquid chromatography XAmide column as two-dimensional chromatography technology for isolation and preparation of coeluting constituents, enzyme-linked immune-sorbent assay as bio-guided assay, and anti-inflammatory bioactivity evaluation for bioactive constituents. A combination of 12 beta-carboline alkaloidswas identified as anti-inflammatory bioactive equivalent combinatorial components from A. kansuensis, which accounts for 1.9% w/w of original A. kansuensis. This work answers the key question of which are real anti-inflammatory components from A. kansuensis and provides a fast and efficient approach for discovering and enriching trace beta-carboline alkaloids from herbal medicines for the first time. More importantly, the discovery of bioactive equivalent combinatorial components could improve the quality control of herbal products and inspire a herbal medicine based on combinatorial therapeutics. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | PICRASMA-QUASSIOIDES ; MEDICINE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000406244500004 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/9794]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Key Lab Tibetan Med Res Qinghai Prov, Xining, Qinghai, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining, Qinghai, Peoples R China 4.Chinese Acad Sci, Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mei, Lijuan,Liu, Zenggen,Cui, Yulei,et al. Anti-inflammatory bioactive equivalence of combinatorial components beta-carboline alkaloids identified in Arenaria kansuensis by two-dimensional chromatography and solid-phase extraction coupled with liquid-liquid extraction enrichment technology[J]. JOURNAL OF SEPARATION SCIENCE,2017,40(14):2895-2905. |
| APA | Mei, Lijuan,Liu, Zenggen,Cui, Yulei,Shen, Na,Dang, Jun,&Tao, Yanduo.(2017).Anti-inflammatory bioactive equivalence of combinatorial components beta-carboline alkaloids identified in Arenaria kansuensis by two-dimensional chromatography and solid-phase extraction coupled with liquid-liquid extraction enrichment technology.JOURNAL OF SEPARATION SCIENCE,40(14),2895-2905. |
| MLA | Mei, Lijuan,et al."Anti-inflammatory bioactive equivalence of combinatorial components beta-carboline alkaloids identified in Arenaria kansuensis by two-dimensional chromatography and solid-phase extraction coupled with liquid-liquid extraction enrichment technology".JOURNAL OF SEPARATION SCIENCE 40.14(2017):2895-2905. |
入库方式： OAI收割
来源：西北高原生物研究所
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