Preparative isolation of flavonoid glycosides from Sphaerophysa salsula using hydrophilic interaction solid-phase extraction coupled with two-dimensional preparative liquid chromatography
文献类型：期刊论文
| 作者 | Wang, Qilan1 ; Tao, Yanduo1; Wang, Weidong1,2; Shao, Yun1; Mei, Lijuan1; Dang, Jun1; Jiao, Lijin1,2
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| 刊名 | JOURNAL OF SEPARATION SCIENCE
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| 出版日期 | 2017-10-01 |
| 卷号 | 40期号:19页码:3808-3816 |
| 关键词 | flavonoid glycosides hydrophilic interaction chromatography hydrophilic interaction solid-phase extraction reversed-phase liquid chromatography traditional Chinese medicine |
| 英文摘要 | An offline preparative two-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography coupled with hydrophilic interaction solid-phase extraction method was developed for the preparative isolation of flavonoid glycosides from a crude sample of Sphaerophysa salsula. First, the non-flavonoids were removed using an XAmide solid-phase extraction cartridge. Based on the separation results of three different chromatographic stationary phases, the first-dimensional preparation was performed on an XAqua C18 prep column, and 15 fractions were obtained from the 5.2 g target sample. Then, three representative fractions were selected for additional purification on an XAmide preparative column to further isolate the flavonoid glycosides. In all, eight flavonoid glycosides were isolated in purities over 97%. The results demonstrated that the two-dimensional liquid chromatography method used in this study was effective for the preparative separation of flavonoid glycosides from Sphaerophysa salsula. Additionally, this method showed great potential for the separation of flavonoid glycosides from other plant materials. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | COUNTER-CURRENT CHROMATOGRAPHY ; FLIGHT MASS-SPECTROMETRY ; ASSISTED EXTRACTION ; PANAX-NOTOGINSENG ; MACROPOROUS RESIN ; SEPARATION ; LEAVES ; IDENTIFICATION ; PURIFICATION ; SAPONINS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000412573400007 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/9809]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Tibetan Med Res, Northwest Inst Plateau Biol, Xining, Qinghai, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Qilan,Tao, Yanduo,Wang, Weidong,et al. Preparative isolation of flavonoid glycosides from Sphaerophysa salsula using hydrophilic interaction solid-phase extraction coupled with two-dimensional preparative liquid chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2017,40(19):3808-3816. |
| APA | Wang, Qilan.,Tao, Yanduo.,Wang, Weidong.,Shao, Yun.,Mei, Lijuan.,...&Jiao, Lijin.(2017).Preparative isolation of flavonoid glycosides from Sphaerophysa salsula using hydrophilic interaction solid-phase extraction coupled with two-dimensional preparative liquid chromatography.JOURNAL OF SEPARATION SCIENCE,40(19),3808-3816. |
| MLA | Wang, Qilan,et al."Preparative isolation of flavonoid glycosides from Sphaerophysa salsula using hydrophilic interaction solid-phase extraction coupled with two-dimensional preparative liquid chromatography".JOURNAL OF SEPARATION SCIENCE 40.19(2017):3808-3816. |
入库方式： OAI收割
来源：西北高原生物研究所
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