Robust hybrid enzyme nanoreactor mediated plasmonic sensing strategy for ultrasensitive screening of anti-diabetic drug
文献类型：期刊论文
| 作者 | Wu, Di1; Hu, Na3 ; Kong, Weiheng2,4; Li, Ning2,4; Dai, Chunji2; Chen, Xuefeng2; Suo, Yourui3; Li, Guoliang2,4; Wu, Yongning1
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| 刊名 | BIOSENSORS & BIOELECTRONICS
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| 出版日期 | 2018-01-15 |
| 卷号 | 99页码:653-659 |
| 关键词 | Hybrid enzyme hierarchical structure alpha-glucosidase Plasmonic sensing Anti-diabetic drug |
| 英文摘要 | Enzyme inhibition based drug screening strategy has been widely employed for new drug discovery. But this strategy faces some challenges in practical application especially for the trace active compound screening from natural products such as the stability of enzyme and the sensitivity of screening approach. Inspired by the above, we for the first time demonstrate the self-assembly of a-glucosidase (GAA) and glucose oxidase (GOx) into one multi-enzymes-inorganic nanoreactor with hierarchical structure (flower shape). The hybrid enzyme nanoreactor enjoys the merits including the character of assembly line, the enhanced enzymatic activity and robust stability. The flower shape of enzyme nanoreactor possessed a bigger specific surface area, facilitating the trace GAA inhibitor detection. Based on the above, we proposed an enzyme nanoreactor mediated plasmonic sensing strategy for anti-diabetic drug screening. First, maltose was chosen as the substrate for GAA and the generated glucose were immediately utilized by GOx to generate H2O2, and finally, H2O2 etched the Ag nanoprism to round nanodiscs, resulting in the blue shift of surface plasmon resonance (SPR) absorption band. With the aid of hybrid enzyme nanoreactor guided SPR, the ultrasensitive screening of GAA inhibitor (i.e. anti-diabetic drug) can be realized with the detection limit of 5 nM for acarbose. The proposed approach was successfully utilized for GAA inhibitor screening from natural products. We anticipate that the proposed sensing method may provide new insights and inspirations in the enzyme inhibition based drug discovery and clinical diagnosis. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
| 研究领域[WOS] | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| 关键词[WOS] | IMMOBILIZATION ; NANOPARTICLES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000413284200084 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/9830]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.China Natl Ctr Food Safety Risk Assessment, Key Labs Chem Safety & Hlth, Beijing 100050, Peoples R China 2.Shaanxi Univ Sci & Technol, Sch Food & Biol Engn, Xian 710021, Shaanxi, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China 4.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Di,Hu, Na,Kong, Weiheng,et al. Robust hybrid enzyme nanoreactor mediated plasmonic sensing strategy for ultrasensitive screening of anti-diabetic drug[J]. BIOSENSORS & BIOELECTRONICS,2018,99:653-659. |
| APA | Wu, Di.,Hu, Na.,Kong, Weiheng.,Li, Ning.,Dai, Chunji.,...&Wu, Yongning.(2018).Robust hybrid enzyme nanoreactor mediated plasmonic sensing strategy for ultrasensitive screening of anti-diabetic drug.BIOSENSORS & BIOELECTRONICS,99,653-659. |
| MLA | Wu, Di,et al."Robust hybrid enzyme nanoreactor mediated plasmonic sensing strategy for ultrasensitive screening of anti-diabetic drug".BIOSENSORS & BIOELECTRONICS 99(2018):653-659. |
入库方式： OAI收割
来源：西北高原生物研究所
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