Wavefront-sensing-based autofocusing in microscopy
文献类型：期刊论文
| 作者 | Meng, Xin; Tian, Xiaolin; Xu, Jing; Wang, Shouyu; Liu, Cheng; Xue, Liang; Liu, Fei; Cui, Haoyang; Gao, Shumei; Kong, Yan |
| 刊名 | J. Biomed. Opt.
 |
| 出版日期 | 2017 |
| 卷号 | 22期号:8 |
| 通讯作者 | cheng.liu@hotmail.co.uk ; wsy_photonics@yahoo.com |
| 英文摘要 | Massive image acquisition is required along the optical axis in the classical image-analysis-based autofocus method, which significantly decreases autofocus efficiency. A wavefront-sensing-based autofocus technique is proposed to increase the speed of autofocusing and obtain high localization accuracy. Intensities at different planes along the optical axis can be computed numerically after extracting the wavefront at defocus position with the help of the transport-of-intensity equation method. According to the focus criterion, the focal plane can then be determined, and after sample shifting to this plane, the in-focus image can be recorded. The proposed approach allows for fast, precise focus detection with fewer image acquisitions compared to classical image-analysis-based autofocus techniques, and it can be applied in commercial microscopes only with an extra illumination filter. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [61705092, 11647144, 31522056]; National Natural Science Foundation of Jiangsu Province of China [BK20130162, BK20170194]; Shanghai Sailing Program [17YF1407000]; Fundamental Research Funds for the Central Universities [JUSRP115A14, JUSRP51721B]; Local Colleges and Universities Capacity Building Program [15110500900, 14110500900] |
| WOS记录号 | WOS:000410739500018 |
| 源URL | [http://ir.siom.ac.cn/handle/181231/28779]  |
| 专题 | 上海光学精密机械研究所_其他 |
| 作者单位 | 中国科学院上海光学精密机械研究所 |
| 推荐引用方式 GB/T 7714 | Meng, Xin,Tian, Xiaolin,Xu, Jing,et al. Wavefront-sensing-based autofocusing in microscopy[J]. J. Biomed. Opt.,2017,22(8). |
| APA | Meng, Xin.,Tian, Xiaolin.,Xu, Jing.,Wang, Shouyu.,Liu, Cheng.,...&Kong, Yan.(2017).Wavefront-sensing-based autofocusing in microscopy.J. Biomed. Opt.,22(8). |
| MLA | Meng, Xin,et al."Wavefront-sensing-based autofocusing in microscopy".J. Biomed. Opt. 22.8(2017). |
入库方式： OAI收割
来源：上海光学精密机械研究所
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