Surface sieving coordinated IMAC material for purification of His-tagged proteins
文献类型：期刊论文
| 作者 | Zhang, Lihua1; Li, Senwu1,2; Yang, Kaiguang1; Liu, Lukuan1,2,3; Zhao, Baofeng1; Chen, Yuanbo1,2; Li, Xiao1; Zhang, Yukui1 |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2017-10-29 |
| 卷号 | 997页码:9-15 |
| 关键词 | Surface sieving Metal ion affinity Protein purification His-tag |
| 英文摘要 | Tailor-made materials for the purification of proteins with His-tag was designed through synergizing the selectivity of surface sieving and metal ion affinity. By excluding impurity proteins out of the surface polymer network, such materials could purify His-tagged proteins from the crude cell lysis with purity up to 90%, improved by 14% compared to that obtained by the commercial metal chelating affinity materials. This study might promote the His-tagged protein purification to a new level. (c) 2017 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | ION AFFINITY-CHROMATOGRAPHY ; RECOMBINANT PROTEINS ; MAGNETIC SEPARATION ; RAFT POLYMERIZATION ; PERSPECTIVES ; NANOPARTICLE ; ADSORPTION ; PROTEOMICS ; STRATEGY ; DELIVERY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000415334500002 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/149669]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Lihua,Li, Senwu,Yang, Kaiguang,et al. Surface sieving coordinated IMAC material for purification of His-tagged proteins[J]. ANALYTICA CHIMICA ACTA,2017,997:9-15. |
| APA | Zhang, Lihua.,Li, Senwu.,Yang, Kaiguang.,Liu, Lukuan.,Zhao, Baofeng.,...&Zhang, Yukui.(2017).Surface sieving coordinated IMAC material for purification of His-tagged proteins.ANALYTICA CHIMICA ACTA,997,9-15. |
| MLA | Zhang, Lihua,et al."Surface sieving coordinated IMAC material for purification of His-tagged proteins".ANALYTICA CHIMICA ACTA 997(2017):9-15. |
入库方式： OAI收割
来源：大连化学物理研究所
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