A Method for Microalgae Proteomics Analysis Based on Modified Filter-Aided Sample Preparation
文献类型：期刊论文
| 作者 | Zhang, Haowei1; Wang, Yan2; Zhu, Zhen1; Tian, Jing1; Xue, Song3; Li, Song1; Cao, Xupeng3 |
| 刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| 出版日期 | 2017-11-01 |
| 卷号 | 183期号:3页码:923-930 |
| 关键词 | FASP mFASP Chlamydomonas reinhardtii Proteomics Sample preparation |
| 英文摘要 | With the fast development of microalgal biofuel researches, the proteomics studies of microalgae increased quickly. A filter-aided sample preparation (FASP) method is widely used proteomics sample preparation method since 2009. Here, a method of microalgae proteomics analysis based on modified filter-aided sample preparation (mFASP) was described to meet the characteristics of microalgae cells and eliminate the error caused by over-alkylation. Using Chlamydomonas reinhardtii as the model, the prepared sample was tested by standard LC-MS/MS and compared with the previous reports. The results showed mFASP is suitable for most of occasions of microalgae proteomics studies. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | CHLAMYDOMONAS-REINHARDTII ; PROTEIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000414195200017 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/149709]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Polytech Univ, Sch Bioengn, Dalian 116034, Peoples R China 2.Chinese Acad Sci, Novel Technol & Mat Separat & Detect Biomol Grp, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Marine Bioengn Grp, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Haowei,Wang, Yan,Zhu, Zhen,et al. A Method for Microalgae Proteomics Analysis Based on Modified Filter-Aided Sample Preparation[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2017,183(3):923-930. |
| APA | Zhang, Haowei.,Wang, Yan.,Zhu, Zhen.,Tian, Jing.,Xue, Song.,...&Cao, Xupeng.(2017).A Method for Microalgae Proteomics Analysis Based on Modified Filter-Aided Sample Preparation.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,183(3),923-930. |
| MLA | Zhang, Haowei,et al."A Method for Microalgae Proteomics Analysis Based on Modified Filter-Aided Sample Preparation".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 183.3(2017):923-930. |
入库方式： OAI收割
来源：大连化学物理研究所
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