Development of an Agrobacterium-Mediated Transformation Method and Evaluation of Two Exogenous Constitutive Promoters in Oleaginous Yeast Lipomyces starkeyi
文献类型：期刊论文
| 作者 | Lin, Xinping2; Liu, Sasa2; Bao, Ruiqi2; Gao, Ning2; Zhang, Sufang1; Zhu, Rongqian2; Zhao, Zongbao Kent1 |
| 刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| 出版日期 | 2017-11-01 |
| 卷号 | 183期号:3页码:867-875 |
| 关键词 | Lipomyces starkeyi Agrobacterium-mediated transformation Promoters Genetic engineering Biofuels |
| 英文摘要 | Oleaginous yeast Lipomyces starkeyi, a promising strain of great biotechnical importance, is able to accumulate over 60% of its cell biomass as triacylglycerols (TAGs). It is promising to directly produce the derivatives of TAGs, such as long-chain fatty acid methyl esters and alkanes, in L. starkeyi. However, techniques for genetic modification of this oleaginous yeast are lacking, thus, further research is needed to develop genetic tools and functional elements. Here, we used two exogenous promoters (pGPD and pPGK) from oleaginous yeast Rhodosporidium toruloides to establish a simpler Agrobacterium-mediated transformation (AMT) method for L. starkeyi. Hygromycin-resistant transformants were obtained on antibiotic-contained plate. Mitotic stability test, genotype verification by PCR, and protein expression confirmation all demonstrated the success of this method. Furthermore, the strength of these two promoters was evaluated at the phenotypic level on a hygromycin-gradient plate and at the transcriptional level by real-time quantitative PCR. The PGK promoter strength was 2.2-fold as that of GPD promoter to initiate the expression of the hygromycin-resistance gene. This study provided an easy and efficient genetic manipulation method and elements of the oleaginous yeast L. starkeyi for constructing superior strains to produce advanced biofuels. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | SACCHAROMYCES-CEREVISIAE ; RHODOSPORIDIUM-TORULOIDES ; LIPID PRODUCTION ; CO-FERMENTATION ; TUMEFACIENS ; INTEGRATION ; EXPRESSION ; GENOMICS ; BIOFUELS ; CLONING |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000414195200013 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/149733]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China 2.Dalian Polytech Univ, Sch Food Sci & Technol, Natl Engn Res Ctr Seafood, Dalian 116034, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, Xinping,Liu, Sasa,Bao, Ruiqi,et al. Development of an Agrobacterium-Mediated Transformation Method and Evaluation of Two Exogenous Constitutive Promoters in Oleaginous Yeast Lipomyces starkeyi[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2017,183(3):867-875. |
| APA | Lin, Xinping.,Liu, Sasa.,Bao, Ruiqi.,Gao, Ning.,Zhang, Sufang.,...&Zhao, Zongbao Kent.(2017).Development of an Agrobacterium-Mediated Transformation Method and Evaluation of Two Exogenous Constitutive Promoters in Oleaginous Yeast Lipomyces starkeyi.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,183(3),867-875. |
| MLA | Lin, Xinping,et al."Development of an Agrobacterium-Mediated Transformation Method and Evaluation of Two Exogenous Constitutive Promoters in Oleaginous Yeast Lipomyces starkeyi".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 183.3(2017):867-875. |
入库方式： OAI收割
来源：大连化学物理研究所
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