An Optimized Two-Photon Fluorescent Probe for Biological Sensing and Imaging of Catechol-O-Methyltransferase
文献类型：期刊论文
| 作者 | Wang, Ping2; Xia, Yang-Liu3; Zou, Li-Wei2; Qian, Xing-Kai3; Dou, Tong-Yi3; Jin, Qiang2; Li, Shi-Yang3; Yu, Yang3; Wang, Dan-Dan2; Luo, Qun1 |
| 刊名 | CHEMISTRY-A EUROPEAN JOURNAL
 |
| 出版日期 | 2017-08-10 |
| 卷号 | 23期号:45页码:10800-10807 |
| 关键词 | bio-imaging biosensing catechol-o-methyltransferase coumarins fluorescence |
| 英文摘要 | A practical two-photon fluorescent probe was developed for highly sensitive and selective sensing of the activities of catechol-O-methyltransferase (COMT) in complex biological samples. To this end, a series of 3-substituted 7,8-dihydroxycoumarins were designed and synthesized. Among them, 3-BTD displayed the best combination of selectivity, sensitivity, reactivity, and fluorescence response following COMT-catalyzed 8-O-methylation. The newly developed two-photon fluorescent probe 3-BTD can be used for determining the activities of COMT in complex biological samples and bio-imaging of endogenous COMT in living cells and tissue slices with good cell permeability, low cytotoxicity, and high imaging resolution. All these findings suggest that 3-BTD holds great promise for developing therapeutic molecules that target COMT, as well as for exploring COMT-associated biological processes and its biological functions in living systems. Furthermore, the strategy also sheds new light on the development of fluorescent probes for other conjugative enzymes. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Multidisciplinary |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | NITRIC-OXIDE ; PARKINSONS-DISEASE ; LIVING CELLS ; METHYLATION ; DESIGN ; INHIBITORS ; COMT ; SCHIZOPHRENIA ; POLYMORPHISM ; DISORDERS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000407397300015 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/149799]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Natl Ctr Mass Spectrometry Beijing, CAS Key Lab Analyt Chem Living Biosyst,Inst Chem, Beijing 100190, Peoples R China 2.Shanghai Univ Tradit Chinese Med, Shanghai 201203, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Ping,Xia, Yang-Liu,Zou, Li-Wei,et al. An Optimized Two-Photon Fluorescent Probe for Biological Sensing and Imaging of Catechol-O-Methyltransferase[J]. CHEMISTRY-A EUROPEAN JOURNAL,2017,23(45):10800-10807. |
| APA | Wang, Ping.,Xia, Yang-Liu.,Zou, Li-Wei.,Qian, Xing-Kai.,Dou, Tong-Yi.,...&Yang, Ling.(2017).An Optimized Two-Photon Fluorescent Probe for Biological Sensing and Imaging of Catechol-O-Methyltransferase.CHEMISTRY-A EUROPEAN JOURNAL,23(45),10800-10807. |
| MLA | Wang, Ping,et al."An Optimized Two-Photon Fluorescent Probe for Biological Sensing and Imaging of Catechol-O-Methyltransferase".CHEMISTRY-A EUROPEAN JOURNAL 23.45(2017):10800-10807. |
入库方式： OAI收割
来源：大连化学物理研究所
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