Chloroplasts Isolation from Chlamydomonas reinhardtii under Nitrogen Stress
文献类型：期刊论文
| 作者 | Chi, Zhan-You3; Xue, Song1; Cao, Xu-Peng1; Fan, Yan1,4; Chu, Ya-Dong1; Xie, Xi2; Jiang, Jun-Peng1,4; Yang, Miao1,3,4 |
| 刊名 | FRONTIERS IN PLANT SCIENCE
 |
| 出版日期 | 2017-08-29 |
| 卷号 | 8 |
| 关键词 | Chlamydomonas chloroplast isolation triacylglycerol nitrogen stress fatty acid biomarkers |
| 英文摘要 | Triacylglycerols are produced in abundance through chloroplast and endoplasmic reticulum pathways in some microalgae exposed to stress, though the relative contribution of either pathway remains elusive. Characterization of these pathways requires isolation of the organelles. In this study, an efficient and reproducible approach, including homogenous batch cultures of nitrogen-deprived algal cells in photobioreactors, gentle cell disruption using a simple custom-made disruptor with mechanical shear force, optimized differential centrifugation and Percoll density gradient centrifugation, was developed to isolate chloroplasts from Chlamydomonas reinhardtii subjected to nitrogen stress. Using this approach, the maximum limited stress duration was 4 h and the stressed cells exhibited 19 and 32% decreases in intracellular chlorophyll and nitrogen content, respectively. Chloroplasts with 48 - 300 mu g chlorophyll were successfully isolated from stressed cells containing 10 mg chlorophyll. These stressed chloroplasts appeared intact, as monitored by ultrastructure observation and a novel quality control method involving the fatty acid biomarkers. This approach can provide sufficient quantities of intact stressed chloroplasts for subcellular biochemical studies in microalgae. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Plant Sciences |
| 研究领域[WOS] | Plant Sciences |
| 关键词[WOS] | INTACT CHLOROPLASTS ; INORGANIC CARBON ; HEAT-STRESS ; LOW CO2 ; CELLS ; BIOSYNTHESIS ; LIPIDS ; PHOTOSYNTHESIS ; FRACTIONATION ; ACCUMULATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000408660800001 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/149974]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioengn Grp, Dalian, Peoples R China 2.Liaoning Ocean & Fisheries Sci Res Inst, Dalian, Peoples R China 3.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chi, Zhan-You,Xue, Song,Cao, Xu-Peng,et al. Chloroplasts Isolation from Chlamydomonas reinhardtii under Nitrogen Stress[J]. FRONTIERS IN PLANT SCIENCE,2017,8. |
| APA | Chi, Zhan-You.,Xue, Song.,Cao, Xu-Peng.,Fan, Yan.,Chu, Ya-Dong.,...&Yang, Miao.(2017).Chloroplasts Isolation from Chlamydomonas reinhardtii under Nitrogen Stress.FRONTIERS IN PLANT SCIENCE,8. |
| MLA | Chi, Zhan-You,et al."Chloroplasts Isolation from Chlamydomonas reinhardtii under Nitrogen Stress".FRONTIERS IN PLANT SCIENCE 8(2017). |
入库方式： OAI收割
来源：大连化学物理研究所
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