Inert Gas Deactivates Protein Activity by Aggregation
文献类型：期刊论文
| 作者 | Zhang, Lijuan4,5; Fang, Haiping4,8; Zhang, Xuehua1; Hu, Jun2,4; Lu, Junhong2,4; Li, Guohui6; Tai, Renzhong4,5; Gao, Yi4,8; Zhang, Meng4,8; Wang, Xingya3,5 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2017-08-31 |
| 卷号 | 7 |
| 英文摘要 | Biologically inert gases play important roles in the biological functionality of proteins. However, researchers lack a full understanding of the effects of these gases since they are very chemically stable only weakly absorbed by biological tissues. By combining X-ray fluorescence, particle sizing and molecular dynamics (MD) simulations, this work shows that the aggregation of these inert gases near the hydrophobic active cavity of pepsin should lead to protein deactivation. Micro X-ray fluorescence spectra show that a pepsin solution can contain a high concentration of Xe or Kr after gassing, and that the gas concentrations decrease quickly with degassing time. Biological activity experiments indicate a reversible deactivation of the protein during this gassing and degassing. Meanwhile, the nanoparticle size measurements reveal a higher number of "nanoparticles" in gas-containing pepsin solution, also supporting the possible interaction between inert gases and the protein. Further, MD simulations indicate that gas molecules can aggregate into a tiny bubble shape near the hydrophobic active cavity of pepsin, suggesting a mechanism for reducing their biological function. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | DER-WAALS COMPLEXES ; XENON ; BINDING ; XE ; MYOGLOBIN ; PATHWAYS ; NITROGEN ; CAVITY ; KR ; IONIZATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000408781200035 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/149979]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.RMIT Univ, Sch Engn, Soft Matter & Interfaces Grp, Melbourne, Vic 3001, Australia 2.Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Key Lab Interfacial Phys & Technol, Shanghai 201800, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Shanghai 201204, Peoples R China 6.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Dalian 116023, Peoples R China 7.Cent South Univ Forestry & Technol, Inst Math & Phys, Changsha 410004, Hunan, Peoples R China 8.Chinese Acad Sci, Shanghai Inst Appl Phys, Div Interfacial Water, Shanghai 201800, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Lijuan,Fang, Haiping,Zhang, Xuehua,et al. Inert Gas Deactivates Protein Activity by Aggregation[J]. SCIENTIFIC REPORTS,2017,7. |
| APA | Zhang, Lijuan.,Fang, Haiping.,Zhang, Xuehua.,Hu, Jun.,Lu, Junhong.,...&Zhang, Yuebin.(2017).Inert Gas Deactivates Protein Activity by Aggregation.SCIENTIFIC REPORTS,7. |
| MLA | Zhang, Lijuan,et al."Inert Gas Deactivates Protein Activity by Aggregation".SCIENTIFIC REPORTS 7(2017). |
入库方式： OAI收割
来源：大连化学物理研究所
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