Characterization the carotenoid productions and profiles of three Rhodosporidium toruloides mutants from Agrobacterium tumefaciens-mediated transformation
文献类型：期刊论文
| 作者 | Su, Yichen1; Lin, Xinping2; Gao, Ning2; Liu, Sasa2; Zhang, Sufang3; Song, Shuang2; Ji, Chaofan2; Dong, Xiuping2; Zhao, Zongbao Kent3; Zhu, Beiwei2 |
| 刊名 | YEAST
 |
| 出版日期 | 2017-08-01 |
| 卷号 | 34期号:8页码:335-342 |
| 关键词 | Rhodosporidium toruloides carotenoids Agrobacterium tumefaciens-mediated transformation T-DNA insertion mutants |
| 英文摘要 | The red yeast Rhodosporidium toruloides is a known lipid producer capable of accumulating large amounts of triacylglycerols and carotenoids. However, it remains challenging to study its carotenoid production profiles owing to limited biochemical information and inefficient genetic tools. Here we used an Agrobacterium tumefaciensmediated transformation (ATMT) to change its carotenoid production and profiles. We constructed R. toruloides NP11 mutant libraries with ATMT, selected three mutants with different colours, characterized their carotenoid products by high-pressure liquid chromatography-mass spectrometry (HPLC-MS) analysis and assured differences among those strains in terms of carotenoid production and its composition profiles. We then located T-DNA insertion sites using the genome walking technology and provided discussions in terms of the new phenotypes. This study is the first of its kind to change the carotenoid production profiles in R. toruloides. Copyright (C) 2017 John Wiley & Sons, Ltd. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Microbiology ; Mycology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Microbiology ; Mycology |
| 关键词[WOS] | BY-PRODUCTS ; RHODOTORULA ; YEASTS ; EFFICIENCY ; GLYCEROL ; LIPIDS ; FOOD |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000409009500002 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/149981]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Oregon State Univ, Seafood Res & Educ Ctr, Astoria, OR 97103 USA 2.Dalian Polytech Univ, Natl Engn Res Ctr Seafood, Sch Food Sci & Technol, Dalian 116034, Peoples R China 3.Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Su, Yichen,Lin, Xinping,Gao, Ning,et al. Characterization the carotenoid productions and profiles of three Rhodosporidium toruloides mutants from Agrobacterium tumefaciens-mediated transformation[J]. YEAST,2017,34(8):335-342. |
| APA | Su, Yichen.,Lin, Xinping.,Gao, Ning.,Liu, Sasa.,Zhang, Sufang.,...&Zhu, Beiwei.(2017).Characterization the carotenoid productions and profiles of three Rhodosporidium toruloides mutants from Agrobacterium tumefaciens-mediated transformation.YEAST,34(8),335-342. |
| MLA | Su, Yichen,et al."Characterization the carotenoid productions and profiles of three Rhodosporidium toruloides mutants from Agrobacterium tumefaciens-mediated transformation".YEAST 34.8(2017):335-342. |
入库方式： OAI收割
来源：大连化学物理研究所
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