Thiol-yne click synthesis of boronic acid functionalized silica nanoparticle-graphene oxide composites for highly selective enrichment of glycoproteins
文献类型：期刊论文
| 作者 | Yang, Jingai1; He, Xiwen1; Chen, Langxing1,2; Zhang, Yukui1,3 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2017-09-01 |
| 卷号 | 1513页码:118-125 |
| 关键词 | Graphene oxide Thiol-yne click reaction Boronate affinity Glycoprotein |
| 英文摘要 | In this work, a facile novel strategy is developed for the preparation of 4-mercapto-phenylboronic acid functionalized silica nanoparticle-graphene oxide composite (GO@MPBA) via thiol-yne click reaction. The morphology, structure and composition of the resulting GO@MPBA was characterized by transmission electron microscopy, X-ray powder diffraction, Fourier transform infrared spectroscopy, Fourier transform Raman spectroscopy, Thermogravimetric analysis and X-ray photoelectron spectrometry. GO@MPBA exhibited the high binding capacity towards glycoproteins such as ovalbumin (1288.8 mg g(-1)), immunoglobulin G (1144.1 mg g(-1)), transferrin (592.1 mg g(-1)) and horseradish peroxidase (392.4 mg g(-1)), in contrast, the binding capacity of each non-glycoproteins (cytochrome c, deoxyribonuclease A, pepsin, trypsin, lysozyme and bovine serum albumin) is less than 50 mg g-1. Furthermore, the GO@MPBA still remained a good binding capacity after the five times of adsorption-desorption cycles. The selective enrichment of glycoproteins from real egg white samples by GO@MPBA was demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. This work could present a facile novel approach of surface modification to design environmentally friendly and more efficient adsorbents for the isolation and enrichment of glycoprotein from complex biological samples. (C) 2017 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | TRANSFER RADICAL POLYMERIZATION ; MOLECULARLY IMPRINTED POLYMERS ; SOLID-PHASE EXTRACTION ; MAGNETIC NANOPARTICLES ; MONOLITHIC COLUMN ; GRAPHITE OXIDE ; LIQUID-CHROMATOGRAPHY ; GLYCOPEPTIDES ; CHEMISTRY ; GLYCOSYLATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000408288400013 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/150003]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin Key Lab Biosensing & Mol Recognit, Res Ctr Analyt Sci,Coll Chem, Tianjin 300071, Peoples R China 2.Nankai Univ, Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Jingai,He, Xiwen,Chen, Langxing,et al. Thiol-yne click synthesis of boronic acid functionalized silica nanoparticle-graphene oxide composites for highly selective enrichment of glycoproteins[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1513:118-125. |
| APA | Yang, Jingai,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2017).Thiol-yne click synthesis of boronic acid functionalized silica nanoparticle-graphene oxide composites for highly selective enrichment of glycoproteins.JOURNAL OF CHROMATOGRAPHY A,1513,118-125. |
| MLA | Yang, Jingai,et al."Thiol-yne click synthesis of boronic acid functionalized silica nanoparticle-graphene oxide composites for highly selective enrichment of glycoproteins".JOURNAL OF CHROMATOGRAPHY A 1513(2017):118-125. |
入库方式： OAI收割
来源：大连化学物理研究所
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