The inhibition of LPS-induced inflammation in RAW264.7 macrophages via the PI3K/Akt pathway by highly N-acetylated chitooligosaccharide
文献类型：期刊论文
| 作者 | Xu, Qingsong2,3; Liu, Meisi3; Liu, Qishun3; Wang, Wenxia3; Du, Yuguang1; Yin, Heng3 |
| 刊名 | CARBOHYDRATE POLYMERS
 |
| 出版日期 | 2017-10-15 |
| 卷号 | 174页码:1138-1143 |
| 关键词 | N-acetylated chitooligosaccharide Lipopolysaccharides Interleukin-6 Tumor necrosis factor-alpha Inflammation PI3K/Akt pathway |
| 英文摘要 | Chitooligosaccharide (COS) has been shown to regulate many biological functions, such as antimicrobial effect and antitumor activity. In the present study, highly N-acetylated chitooligosaccharide(NACOS) was prepared by N-acetylation of COS, and the anti-inflammatory activity of NACOS in macrophages were evaluated. The results indicated NACOS significantly suppressed the LPS-induced proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-alpha(TNF-alpha) expression. Furthermore, the increased levels of reactive oxygen species (ROS) and nitric oxide (NO) were repressed by NACOS in a dose dependent manner. However, NACOS itself had no significant effect on the cell viability and cellular morphology. Signal transduction studies demonstrated that NACOS remarkably inhibited LPS-enhanced phosphorylation of phosphatidylinositol 3-kinase (PI3K) and Akt. These findings provide a possible molecular mechanism by which NACOS inhibit LPS-induced inflammatory response in macrophages, and a basis for utilizing NACOS in pharmaceutical therapy against inflammation. (C) 2017 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Applied ; Chemistry, Organic ; Polymer Science |
| 研究领域[WOS] | Chemistry ; Polymer Science |
| 关键词[WOS] | RAW 264.7 CELLS ; CHITOSAN OLIGOSACCHARIDES ; IN-VITRO ; ENDOTHELIAL-CELLS ; MOLECULAR-WEIGHT ; CHITIN ; EXPRESSION ; MICROGLIA ; DISEASES ; VIVO |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000407696800119 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/150006]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China 2.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, 457 Zhongshan Rd, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Qingsong,Liu, Meisi,Liu, Qishun,et al. The inhibition of LPS-induced inflammation in RAW264.7 macrophages via the PI3K/Akt pathway by highly N-acetylated chitooligosaccharide[J]. CARBOHYDRATE POLYMERS,2017,174:1138-1143. |
| APA | Xu, Qingsong,Liu, Meisi,Liu, Qishun,Wang, Wenxia,Du, Yuguang,&Yin, Heng.(2017).The inhibition of LPS-induced inflammation in RAW264.7 macrophages via the PI3K/Akt pathway by highly N-acetylated chitooligosaccharide.CARBOHYDRATE POLYMERS,174,1138-1143. |
| MLA | Xu, Qingsong,et al."The inhibition of LPS-induced inflammation in RAW264.7 macrophages via the PI3K/Akt pathway by highly N-acetylated chitooligosaccharide".CARBOHYDRATE POLYMERS 174(2017):1138-1143. |
入库方式： OAI收割
来源：大连化学物理研究所
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