High-throughput metabolic profiling based on small amount of hepatic cells
文献类型：期刊论文
| 作者 | Zhou, Lina; Yin, Peiyuan; Piao, Hailong; Lu, Xin; Xu, Guowang; Wang, Zhichao; Tang, Ling; Luo, Ping; Gao, Peng |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2017-09-01 |
| 卷号 | 38期号:18页码:2296-2303 |
| 关键词 | A small amount of cells Amino acid metabolism High throughput Metabolic profiling |
| 英文摘要 | Common metabolomics platforms require about 10(6) cells, which has a limited throughput due to the time-consuming steps of cell culture and preparation. There is a demand for metabolic profiling methods to improve analytical throughput and detection sensitivity based on small amount of cells. In this study, we proposed a high-throughput scheme, integrating 96-well plate cell cultivation, in-situ cell pretreatment, and sensitive dansylation labeling coupled with LC-MS analysis of metabolites inside HepG2 cells (of the order of magnitude of 10(3) cells in each well). A simple and rapid cell pretreatment was performed showing good extraction efficiency and good precision (the RSDs smaller than 5%) for polar metabolites. The recovery in metabolite extraction evaluated with three isotope-labeled amino acids was from 89.7 to 106.3% at low, medium, and high concentrations. The suitability of the method was illustrated by exploring influences of different fatty acids on HepG2 cells. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | EMBRYONIC STEM-CELLS ; FATTY-ACIDS ; DRUG DEVELOPMENT ; CANCER ; CULTURE ; CATABOLISM ; TOXICITY ; ONCOLOGY ; GLYCINE ; DEATH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000410758400007 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/150019]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Lina,Yin, Peiyuan,Piao, Hailong,et al. High-throughput metabolic profiling based on small amount of hepatic cells[J]. ELECTROPHORESIS,2017,38(18):2296-2303. |
| APA | Zhou, Lina.,Yin, Peiyuan.,Piao, Hailong.,Lu, Xin.,Xu, Guowang.,...&Gao, Peng.(2017).High-throughput metabolic profiling based on small amount of hepatic cells.ELECTROPHORESIS,38(18),2296-2303. |
| MLA | Zhou, Lina,et al."High-throughput metabolic profiling based on small amount of hepatic cells".ELECTROPHORESIS 38.18(2017):2296-2303. |
入库方式： OAI收割
来源：大连化学物理研究所
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