Strategies for large-scale analysis of non-histone protein methylation by LC-MS/MS
文献类型：期刊论文
| 作者 | Wang, Qi1,2; Wang, Keyun1,2; Ye, Mingliang1,2 |
| 刊名 | ANALYST
 |
| 出版日期 | 2017-10-17 |
| 卷号 | 142期号:19页码:3536-3548 |
| 英文摘要 | Protein methylation is an important post-translational modification (PTM) that plays crucial roles in the regulation of diverse biological processes. Though many efforts have been devoted to the investigation of protein methylation, the analysis of non-histone methylation at the proteome level is still a great challenge. The alteration of the protein/peptide physicochemical properties caused by methylation is very small, thus it is difficult to develop highly efficient enrichment approaches to separate methylated peptides from a pool of diverse background peptides. The mass shifts caused by methylations are identical to the substitutions of some amino acids, thus it is difficult to confidently identify methylated peptides. In this review, we report on recent advances in the development of methods for large-scale analysis of nonhistone protein methylation. Especially the methods for efficient enrichment and the approaches for controlling identification confidence have been covered. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | ADENOSYL-L-METHIONINE ; AFFINITY-CHROMATOGRAPHY ADSORBENT ; POSITIVE-ION MODE ; MASS-SPECTROMETRY ; LYSINE-METHYLATION ; ARGININE METHYLATION ; PROTEOMIC ANALYSIS ; POSTTRANSLATIONAL MODIFICATIONS ; HUMAN-CELLS ; IN-VIVO |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000411703800001 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/150048]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Qi,Wang, Keyun,Ye, Mingliang. Strategies for large-scale analysis of non-histone protein methylation by LC-MS/MS[J]. ANALYST,2017,142(19):3536-3548. |
| APA | Wang, Qi,Wang, Keyun,&Ye, Mingliang.(2017).Strategies for large-scale analysis of non-histone protein methylation by LC-MS/MS.ANALYST,142(19),3536-3548. |
| MLA | Wang, Qi,et al."Strategies for large-scale analysis of non-histone protein methylation by LC-MS/MS".ANALYST 142.19(2017):3536-3548. |
入库方式： OAI收割
来源：大连化学物理研究所
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