Preparation and optimization of calcium pectate beads for cell encapsulation
文献类型：期刊论文
| 作者 | Zhao, Shan1,3,4; Zhang, Ying4; Liu, Yang4; Yang, Fan2; Xiu, Zhilong1; Ma, Xiaojun2; Sun, Guangwei4 |
| 刊名 | JOURNAL OF APPLIED POLYMER SCIENCE
 |
| 出版日期 | 2017-10-29 |
| 卷号 | 135期号:2 |
| 关键词 | biomaterials biomedical applications gels |
| 英文摘要 | A variety of natural polymers are considered suitable for cell entrapment. Pectin, a natural polysaccharide found in the cell walls of all higher terrestrial plants, presents great potential for cell encapsulation because of its ability to form gels. In this study, we investigated the influences of intrinsic and extrinsic factors on the formation and properties of calcium pectate (CP). Beads of CP prepared with 30-35% methyl esterification presented improved gel properties. The molecular weight (M-w), calcium content, and gel time did not significantly affect the gel properties. The gelling properties, sphericity factor (SF), and swelling ratio were significantly influenced by the degree of methyl esterification (DE) and pectin concentration. The DE (<40%), M-w, and calcium concentration had negative effects on the SFs of CP beads. Moreover, increases in the DE (<40%) and pectin concentration increased the swelling ratio. The C3A cells encapsulated in optimized CP beads maintained good viability and proliferation for up to 2 weeks. In conclusion, CP beads are a potential encapsulation material for cell immobilization and application in related fields. (c) 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 45685. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Polymer Science |
| 研究领域[WOS] | Polymer Science |
| 关键词[WOS] | LOW-METHOXYL-PECTIN ; EGG-BOX MODEL ; GEL BEADS ; BIOMEDICAL APPLICATIONS ; CA2+-PECTIN GELS ; DIVALENT-CATIONS ; DRUG-DELIVERY ; CITRUS PECTIN ; FT-IR ; ALGINATE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000411806400024 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/150049]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, Sci Res Ctr Translat Med, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Shan,Zhang, Ying,Liu, Yang,et al. Preparation and optimization of calcium pectate beads for cell encapsulation[J]. JOURNAL OF APPLIED POLYMER SCIENCE,2017,135(2). |
| APA | Zhao, Shan.,Zhang, Ying.,Liu, Yang.,Yang, Fan.,Xiu, Zhilong.,...&Sun, Guangwei.(2017).Preparation and optimization of calcium pectate beads for cell encapsulation.JOURNAL OF APPLIED POLYMER SCIENCE,135(2). |
| MLA | Zhao, Shan,et al."Preparation and optimization of calcium pectate beads for cell encapsulation".JOURNAL OF APPLIED POLYMER SCIENCE 135.2(2017). |
入库方式： OAI收割
来源：大连化学物理研究所
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