Aptamer-immobilized open tubular capillary column to capture circulating tumor cells for proteome analysis
文献类型：期刊论文
| 作者 | Zhang, Yukui1; Liu, Lukuan1,2,3; Yang, Kaiguang1; Zhu, Xudong1,2; Liang, Yu1; Chen, Yuanbo1,2; Fang, Fei1,2; Zhao, Qun1; Zhang, Lihua1 |
| 刊名 | TALANTA
 |
| 出版日期 | 2017-12-01 |
| 卷号 | 175页码:189-193 |
| 关键词 | Circulating tumor cells Aptamer Proteome Open tubular column |
| 英文摘要 | Circulating tumor cells hold the key to predicting the prognosis and discovering the therapeutic targets. Herein, we proposed a strategy to develop an aptamer-immobilized open tubular capillary column by which SMMC-7721 human hepatoma cells (SMMC-7721 cells) could be captured with an over 70% of capture efficiency and a 3.0 +/- 0.2 of enrichment factor. Owing to the compatibility of the column, the captured cells by the column could be analyzed by LC-MS from protein level and 5 unique proteins of SMMC-7721 cells were identified which could be used as markers to identify SMMC-7721 cells when Jurkat T-leukemia cells (Jurkat cells) were employed as interfering cells. As the key component, the aptamer-immobilized column had the potential to be integrated into the platform for separating, enriching and characterizing rare cells simultaneously. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | MULTIPLE TYPES ; NANOPARTICLES ; ENRICHMENT ; CANCER ; ATTACHMENT ; CHALLENGES ; SEPARATION ; SURFACES ; POLYMER |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000412033100024 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/150063]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yukui,Liu, Lukuan,Yang, Kaiguang,et al. Aptamer-immobilized open tubular capillary column to capture circulating tumor cells for proteome analysis[J]. TALANTA,2017,175:189-193. |
| APA | Zhang, Yukui.,Liu, Lukuan.,Yang, Kaiguang.,Zhu, Xudong.,Liang, Yu.,...&Zhang, Lihua.(2017).Aptamer-immobilized open tubular capillary column to capture circulating tumor cells for proteome analysis.TALANTA,175,189-193. |
| MLA | Zhang, Yukui,et al."Aptamer-immobilized open tubular capillary column to capture circulating tumor cells for proteome analysis".TALANTA 175(2017):189-193. |
入库方式： OAI收割
来源：大连化学物理研究所
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