Real-Time Tracking the Synthesis and Degradation of Albumin in Complex Biological Systems with a near-Infrared Fluorescent Probe
文献类型：期刊论文
| 作者 | Zhang, Yi2; Cui, Jing-Nan5; Guo, Wen-Zhi2; Yang, Ling3; Ge, Guang-Bo3,4; Jin, Qiang1,2,3,4; Feng, Lei5,6; Zhang, Shui-Jun1,2; Wang, Dan-Dan3; Wang, Fang-Jun4 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2017-09-19 |
| 卷号 | 89期号:18页码:9884-9891 |
| 英文摘要 | In this study, a novel fluorescent detection system for biological sensing of human albumin (HA) was developed on the basis of the pseudoesterase activity and substrate preference of HA. The designed near-infrared (NIR) fluorescent probe (DDAP) could be effectively hydrolyzed by HA, accompanied by significant changes in both color and fluorescence spectrum. The sensing mechanism was fully investigated by fluorescence spectroscopy, NMR, and mass spectra. DDAP exhibited excellent selectivity and sensitivity toward HA over a variety of human plasma proteins, hydrolases, and abundant biomolecules found in human body. The probe has been successfully applied to measure native HA in diluted plasma samples and the secreted HA in the hepatocyte culture supernatant. DDAP has also been used for fluorescence imaging of HA reabsorption in living renal cells, and the results show that the probe exhibits good cell permeability, low cytotoxicity and high imaging resolution. Furthermore, DDAP has been successfully used for real-time tracking the uptaking and degradation of albumin in ex vivo mouse kidney models for the first time. All these results clearly demonstrated that DDAP-based assay held great promise for real-time sensing and tracking HA in complex biological systems, which would be very useful for basic researches and clinical diagnosis of HA associated diseases. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | HUMAN SERUM-ALBUMIN ; PSEUDO-ESTERASE ACTIVITY ; HUMAN CARBOXYLESTERASE 1 ; OFF-ON PROBE ; IN-VIVO ; ENZYMATIC-PROPERTIES ; BROMCRESOL PURPLE ; PRACTICAL ASPECTS ; BINDING ; NITROREDUCTASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000411549100040 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/150069]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Zhengzhou Univ, Key Lab Hepatobiliary & Pancreat Surg & Digest Or, Affiliated Hosp 1, Zhengzhou 450001, Henan, Peoples R China 2.Zhengzhou Univ, Dept Hepatobiliary & Pancreat Surg, Affiliated Hosp 1, Zhengzhou 450001, Henan, Peoples R China 3.Shanghai Univ Tradit Chinese Med, Inst Interdisciplinary Med, Shanghai 201213, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 5.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China 6.Dalian Med Univ, Acad Integrat Med, Coll Pharm, Dalian 116044, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yi,Cui, Jing-Nan,Guo, Wen-Zhi,et al. Real-Time Tracking the Synthesis and Degradation of Albumin in Complex Biological Systems with a near-Infrared Fluorescent Probe[J]. ANALYTICAL CHEMISTRY,2017,89(18):9884-9891. |
| APA | Zhang, Yi.,Cui, Jing-Nan.,Guo, Wen-Zhi.,Yang, Ling.,Ge, Guang-Bo.,...&Wang, Fang-Jun.(2017).Real-Time Tracking the Synthesis and Degradation of Albumin in Complex Biological Systems with a near-Infrared Fluorescent Probe.ANALYTICAL CHEMISTRY,89(18),9884-9891. |
| MLA | Zhang, Yi,et al."Real-Time Tracking the Synthesis and Degradation of Albumin in Complex Biological Systems with a near-Infrared Fluorescent Probe".ANALYTICAL CHEMISTRY 89.18(2017):9884-9891. |
入库方式： OAI收割
来源：大连化学物理研究所
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