MicroRNA-205-5p regulates the chemotherapeutic resistance of hepatocellular carcinoma cells by targeting PTEN/JNK/ANXA3 pathway
文献类型：期刊论文
| 作者 | Sun, De-Guang1; Fan, Rong3; Wang, Li-Ming1; Shao, Ping1; Qu, Wei-Kun1; Wang, Cheng-Ye1; Gao, Zhen-Ming1; Tian, Yu1; Sui, Ji-Dong1; Ye, Ming-Liang2 |
| 刊名 | AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
 |
| 出版日期 | 2017 |
| 卷号 | 9期号:9页码:4300-+ |
| 关键词 | MicroRNA-205 hepatocellular carcinoma chemotherapeutic resistance PTEN |
| 英文摘要 | Hepatocellular carcinoma (HCC) is a common malignant tumor of the digestive system, and patients with advanced HCC have a poor outlook, partly due to resistance to chemotherapeutic drugs. Previous studies have implicated microRNAs in the regulation of chemoresistance, and we have previously shown that microRNA (miR)-205- 5p is down-regulated in multiple hepatoma cell lines. Here, we investigate whether miR-205-5p is involved in chemotherapeutic resistance in HCC. Expression of miR-205-5p was measured by real-time quantitative reverse transcription PCR and cell viability was determined using a CCK-8 cell viability assay. Expression of proteins in the PTEN/JNK/ANXA3 pathway were assessed via Western blotting. We found that miR-205-5p expression was down-regulated in all HCC cell lines investigated. In addition, miR-205-5p expression was upregulated by 5-fluorouracil (5-Fu) treatment in Bel-7402 (Bel) cells. Interestingly, miR-205-5p expression was increased in multidrug-resistant Bel-7402/5-Fu (Bel/Fu) cells, compared with Bel cells. We next demonstrated that sensitivity to 5-Fu was increased in Bel/Fu cells after treatment with a miR-205-5p inhibitor. Similarly, increased resistance to 5-Fu was observed in Bel cells after transfection with a miR-205-5p mimic. We injected nude mice with Bel/5-Fu cells to promote tumor growth, and found that co-treatment with a miR-205-5p antagomir and 5-Fu slowed tumor growth more than either treatment alone. Finally, we found that these effects were all associated with changes in the PTEN/JNK/ANXA3 pathway. In conclusion, inhibition of miR-205-5p may reverse chemotherapeutic resistance to 5-Fu, and this may occur via the PTEN/JNK/ANXA3 pathway. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Oncology ; Medicine, Research & Experimental |
| 研究领域[WOS] | Oncology ; Research & Experimental Medicine |
| 关键词[WOS] | COLORECTAL-CANCER CELLS ; TUMOR-SUPPRESSOR ; MIR-205 ; PTEN ; PROLIFERATION ; EXPRESSION ; PROMOTES ; PROTEIN ; GROWTH ; CHEMORESISTANCE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000412145400038 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/150104]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Med Univ, Affiliated Hosp 2, Dept Surg, Div Hepatobiliary & Pancreat Surg, 467 Zhongshan Rd, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China 3.Dalian Med Univ, Affiliated Hosp 2, VIP Ward 2, 467 Zhongshan Rd, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, De-Guang,Fan, Rong,Wang, Li-Ming,et al. MicroRNA-205-5p regulates the chemotherapeutic resistance of hepatocellular carcinoma cells by targeting PTEN/JNK/ANXA3 pathway[J]. AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH,2017,9(9):4300-+. |
| APA | Sun, De-Guang.,Fan, Rong.,Wang, Li-Ming.,Shao, Ping.,Qu, Wei-Kun.,...&Ye, Ming-Liang.(2017).MicroRNA-205-5p regulates the chemotherapeutic resistance of hepatocellular carcinoma cells by targeting PTEN/JNK/ANXA3 pathway.AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH,9(9),4300-+. |
| MLA | Sun, De-Guang,et al."MicroRNA-205-5p regulates the chemotherapeutic resistance of hepatocellular carcinoma cells by targeting PTEN/JNK/ANXA3 pathway".AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH 9.9(2017):4300-+. |
入库方式： OAI收割
来源：大连化学物理研究所
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