A naphthalimide-derived fluorogenic probe for SNAP-tag with a fast record labeling rate
文献类型:期刊论文
作者 | Xu, Zhaochao2; Qiao, Qinglong1,2; Liu, Wenjuan2; Chen, Jie2; Zhou, Wei1,2; Yin, Wenting2; Miao, Lu2; Cui, Jingnan1 |
刊名 | DYES AND PIGMENTS
![]() |
出版日期 | 2017-12-01 |
卷号 | 147页码:327-333 |
关键词 | Fluorogenic probe SNAP-tag Labeling rate Protein imaging Wash free |
英文摘要 | SNAP-tag is one of most popular genetically encoded protein tags that can be labeled with fluorescent molecules for visualizing a protein of interest in live cells. Fluorogenic probes keep dark until they label protein tags, significantly improving the signal-to-noise ratio to image proteins without wash-out step. However, most of reported fluorogenic probes for SNAP-tag suffered from the low or mild labeling rate comparing with non-fluorogenic ones. In this paper, we reported a 4-amino-naphthliamide derived fluorogenic probe for SNAP-tag, which exhibited the fast record labeling rate among fluorogenic probes. Finally, we applied this probe to image proteins in mitochondria and nucleus in live cells without washout steps. (C) 2017 Elsevier Ltd. All rights reserved. |
WOS标题词 | Science & Technology ; Physical Sciences ; Technology |
类目[WOS] | Chemistry, Applied ; Engineering, Chemical ; Materials Science, Textiles |
研究领域[WOS] | Chemistry ; Engineering ; Materials Science |
关键词[WOS] | LIVING CELLS ; FLUORESCENT-PROBE ; PROTEIN ; FLUOROPHORE |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000412612400037 |
源URL | [http://cas-ir.dicp.ac.cn/handle/321008/150105] ![]() |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | 1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
推荐引用方式 GB/T 7714 | Xu, Zhaochao,Qiao, Qinglong,Liu, Wenjuan,et al. A naphthalimide-derived fluorogenic probe for SNAP-tag with a fast record labeling rate[J]. DYES AND PIGMENTS,2017,147:327-333. |
APA | Xu, Zhaochao.,Qiao, Qinglong.,Liu, Wenjuan.,Chen, Jie.,Zhou, Wei.,...&Cui, Jingnan.(2017).A naphthalimide-derived fluorogenic probe for SNAP-tag with a fast record labeling rate.DYES AND PIGMENTS,147,327-333. |
MLA | Xu, Zhaochao,et al."A naphthalimide-derived fluorogenic probe for SNAP-tag with a fast record labeling rate".DYES AND PIGMENTS 147(2017):327-333. |
入库方式: OAI收割
来源:大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。