中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
A naphthalimide-derived fluorogenic probe for SNAP-tag with a fast record labeling rate

文献类型:期刊论文

作者Xu, Zhaochao2; Qiao, Qinglong1,2; Liu, Wenjuan2; Chen, Jie2; Zhou, Wei1,2; Yin, Wenting2; Miao, Lu2; Cui, Jingnan1
刊名DYES AND PIGMENTS
出版日期2017-12-01
卷号147页码:327-333
关键词Fluorogenic probe SNAP-tag Labeling rate Protein imaging Wash free
英文摘要SNAP-tag is one of most popular genetically encoded protein tags that can be labeled with fluorescent molecules for visualizing a protein of interest in live cells. Fluorogenic probes keep dark until they label protein tags, significantly improving the signal-to-noise ratio to image proteins without wash-out step. However, most of reported fluorogenic probes for SNAP-tag suffered from the low or mild labeling rate comparing with non-fluorogenic ones. In this paper, we reported a 4-amino-naphthliamide derived fluorogenic probe for SNAP-tag, which exhibited the fast record labeling rate among fluorogenic probes. Finally, we applied this probe to image proteins in mitochondria and nucleus in live cells without washout steps. (C) 2017 Elsevier Ltd. All rights reserved.
WOS标题词Science & Technology ; Physical Sciences ; Technology
类目[WOS]Chemistry, Applied ; Engineering, Chemical ; Materials Science, Textiles
研究领域[WOS]Chemistry ; Engineering ; Materials Science
关键词[WOS]LIVING CELLS ; FLUORESCENT-PROBE ; PROTEIN ; FLUOROPHORE
收录类别SCI
语种英语
WOS记录号WOS:000412612400037
源URL[http://cas-ir.dicp.ac.cn/handle/321008/150105]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
作者单位1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Xu, Zhaochao,Qiao, Qinglong,Liu, Wenjuan,et al. A naphthalimide-derived fluorogenic probe for SNAP-tag with a fast record labeling rate[J]. DYES AND PIGMENTS,2017,147:327-333.
APA Xu, Zhaochao.,Qiao, Qinglong.,Liu, Wenjuan.,Chen, Jie.,Zhou, Wei.,...&Cui, Jingnan.(2017).A naphthalimide-derived fluorogenic probe for SNAP-tag with a fast record labeling rate.DYES AND PIGMENTS,147,327-333.
MLA Xu, Zhaochao,et al."A naphthalimide-derived fluorogenic probe for SNAP-tag with a fast record labeling rate".DYES AND PIGMENTS 147(2017):327-333.

入库方式: OAI收割

来源:大连化学物理研究所

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