Selective separation of xanthones and saponins from the rhizomes of Anemarrhena asphodeloides by modulating the density of surface charges in C18-bonded stationary phases
文献类型：期刊论文
| 作者 | Feng, Jiatao2; Cai, Jianfeng1; Xin, Huaxia1; Cheng, Lingping1; Guo, Zhimou2; Fu, Qing1; Jin, Yu1; Liang, Xinmiao1,2 |
| 刊名 | ANALYTICAL METHODS
 |
| 出版日期 | 2017-10-14 |
| 卷号 | 9期号:38页码:5604-5610 |
| 英文摘要 | A rapid and robust separation method based on the positively charged reversed-phase (PGRP) stationary phase was developed for selective separation of saponins and xanthones from the rhizomes of Anemarrhena asphodeloides (A. asphodeloides). In this work, the chromatographic performances of three PGRP stationary phases with different surface positive charge densities were systematically evaluated by studying hydrophobicity, effects of pH and buffer concentration of the mobile phase, ion-exchange capacity, etc. The PGRP stationary phase exhibited reversed-phase/anion-exchange mixed-mode properties. Then the retention behaviors of xanthones were investigated. A good retention of xanthones was obtained at high pH and xanthones were easily eluted at low pH. The pH is like an on-off switch on the PGRP stationary phase that controls the retention of xanthones. Finally, this PGRP material was successfully applied to the selective separation of saponins and xanthones from A. asphodeloides. The result demonstrated that neutral and ionic compounds, such as xanthones and saponins from the rhizomes of A. asphodeloides, were separated selectively by modulating both the density of surface charges in the PGRP stationary phase and the pH of the mobile phase. |
| WOS标题词 | Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Chemistry, Analytical ; Food Science & Technology ; Spectroscopy |
| 研究领域[WOS] | Chemistry ; Food Science & Technology ; Spectroscopy |
| 关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; IONIZABLE COMPOUNDS ; STEROIDAL SAPONINS ; ADSORPTION PROCESS ; MASS-SPECTROMETRY ; IONIC-STRENGTH ; PH ; PURIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000412945300006 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/150222]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.East China Univ Sci & Technol, Sch Pharm, Minist Educ, Engn Res Ctr Pharmaceut Proc Chem, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Jiatao,Cai, Jianfeng,Xin, Huaxia,et al. Selective separation of xanthones and saponins from the rhizomes of Anemarrhena asphodeloides by modulating the density of surface charges in C18-bonded stationary phases[J]. ANALYTICAL METHODS,2017,9(38):5604-5610. |
| APA | Feng, Jiatao.,Cai, Jianfeng.,Xin, Huaxia.,Cheng, Lingping.,Guo, Zhimou.,...&Liang, Xinmiao.(2017).Selective separation of xanthones and saponins from the rhizomes of Anemarrhena asphodeloides by modulating the density of surface charges in C18-bonded stationary phases.ANALYTICAL METHODS,9(38),5604-5610. |
| MLA | Feng, Jiatao,et al."Selective separation of xanthones and saponins from the rhizomes of Anemarrhena asphodeloides by modulating the density of surface charges in C18-bonded stationary phases".ANALYTICAL METHODS 9.38(2017):5604-5610. |
入库方式： OAI收割
来源：大连化学物理研究所
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