Deletion of acetate transporter gene ADY2 improved tolerance of Saccharomyces cerevisiae against multiple stresses and enhanced ethanol production in the presence of acetic acid
文献类型：期刊论文
| 作者 | Zhang, Keyu1,2; Mehmood, Muhammad Aamer1,2,5; Zhao, Zongbao Kent4; Bai, Fengwu1,2,3; Zhao, Xinqing1,2; Zhang, Mingming3 |
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2017-12-01 |
| 卷号 | 245页码:1461-1468 |
| 关键词 | Saccharomyces cerevisiae Acetic acid stress tolerance Zinc sulfate Ady2p Ethanol production |
| 英文摘要 | The aim of this work was to study the effects of deleting acetate transporter gene ADY2 on growth and fermentation of Saccharomyces cerevisiae in the presence of inhibitors. Comparative transcriptome analysis revealed that three genes encoding plasma membrane carboxylic acid transporters, especially ADY2, were significantly downregulated under the zinc sulfate addition condition in the presence of acetic acid stress, and the deletion of ADY2 improved growth of S. cerevisiae under acetic acid, ethanol and hydrogen peroxide stresses. Consistently, a concomitant increase in ethanol production by 14.7% in the presence of 3.6 g/L acetic acid was observed in the ADY2 deletion mutant of S. cerevisiae BY4741. Decreased intracellular acetic acid, ROS accumulation, and plasma membrane permeability were observed in the ADY2 deletion mutant. These findings would be useful for developing robust yeast strains for efficient ethanol production. (C) 2017 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 研究领域[WOS] | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 关键词[WOS] | SELF-FLOCCULATING YEAST ; PROGRAMMED CELL-DEATH ; TRANSCRIPTOME ANALYSIS ; LABORATORY EVOLUTION ; IDENTIFICATION ; ZINC ; OVEREXPRESSION ; FERMENTATION ; RESISTANCE ; SUPPLEMENTATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000413038300021 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/150223]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200240, Peoples R China 3.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China 5.Govt Coll Univ Faisalabad, Dept Bioinformat & Biotechnol, Bioenergy Res Ctr, Faisalabad 38000, Pakistan |
| 推荐引用方式 GB/T 7714 | Zhang, Keyu,Mehmood, Muhammad Aamer,Zhao, Zongbao Kent,et al. Deletion of acetate transporter gene ADY2 improved tolerance of Saccharomyces cerevisiae against multiple stresses and enhanced ethanol production in the presence of acetic acid[J]. BIORESOURCE TECHNOLOGY,2017,245:1461-1468. |
| APA | Zhang, Keyu,Mehmood, Muhammad Aamer,Zhao, Zongbao Kent,Bai, Fengwu,Zhao, Xinqing,&Zhang, Mingming.(2017).Deletion of acetate transporter gene ADY2 improved tolerance of Saccharomyces cerevisiae against multiple stresses and enhanced ethanol production in the presence of acetic acid.BIORESOURCE TECHNOLOGY,245,1461-1468. |
| MLA | Zhang, Keyu,et al."Deletion of acetate transporter gene ADY2 improved tolerance of Saccharomyces cerevisiae against multiple stresses and enhanced ethanol production in the presence of acetic acid".BIORESOURCE TECHNOLOGY 245(2017):1461-1468. |
入库方式： OAI收割
来源：大连化学物理研究所
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