Enhancing the Mass Spectrometry Sensitivity for Oligonucleotide Detection by Organic Vapor Assisted Electrospray
文献类型：期刊论文
| 作者 | Zhang, Yukui1; Weng, Guofeng1,2; Liu, Zheyi1,3; Chen, Jin1,3; Wang, Fangjun1; Pan, Yuanjiang2 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2017-10-03 |
| 卷号 | 89期号:19页码:10256-10263 |
| 英文摘要 | There are two challenges in oligonucleotide detection by liquid chromatography coupled with mass spectrometry (LC-MS), the serious ion suppression effects caused by ion-pair reagents and the low detection sensitivity in positive mode MS. In this study, highly concentrated alcohol vapors were introduced into an enclosed electrospray ionization chamber, and oligonucleotides could be well detected in negative mode MS even with 100 mM triethylammonium acetate (TEAA) as an ion-pair reagent. The MS signal intensity was improved 600-fold (for standard oligonucleotide dT15) by the isopropanol vapor assisted electrospray, and effective ion-pair LC separation was feasibly coupled with high-sensitive MS detection. Then, oligonucleotides were successfully detected in positive mode MS with few adducts by propanoic acid vapor assisted electrospray. The signal intensity was enhanced more than 10-fold on average compared with adding acids into the electrospray solution. Finally, oligonucleotides and peptides or histones were simultaneously detected in MS with little interference with each other. Our strategy provides a useful alternative for investigating the biological functions of oligonucleotides. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | CHEMICALLY-MODIFIED OLIGONUCLEOTIDES ; COLLISION-INDUCED DISSOCIATION ; LIQUID-CHROMATOGRAPHY ; NUCLEIC-ACIDS ; POSTTRANSLATIONAL MODIFICATIONS ; DNA OLIGONUCLEOTIDES ; PROTEOME ANALYSIS ; CHARGED DROPLETS ; CATION-EXCHANGE ; IONIZATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000412716800023 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/150227]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Zhejiang Univ, Dept Chem, Hangzhou 310027, Zhejiang, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yukui,Weng, Guofeng,Liu, Zheyi,et al. Enhancing the Mass Spectrometry Sensitivity for Oligonucleotide Detection by Organic Vapor Assisted Electrospray[J]. ANALYTICAL CHEMISTRY,2017,89(19):10256-10263. |
| APA | Zhang, Yukui,Weng, Guofeng,Liu, Zheyi,Chen, Jin,Wang, Fangjun,&Pan, Yuanjiang.(2017).Enhancing the Mass Spectrometry Sensitivity for Oligonucleotide Detection by Organic Vapor Assisted Electrospray.ANALYTICAL CHEMISTRY,89(19),10256-10263. |
| MLA | Zhang, Yukui,et al."Enhancing the Mass Spectrometry Sensitivity for Oligonucleotide Detection by Organic Vapor Assisted Electrospray".ANALYTICAL CHEMISTRY 89.19(2017):10256-10263. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。