Au-cysteine modified macroporous adsorption resin: preparation and highly selective enrichment and identification of N-linked glycopeptides from the complex biological sample
文献类型：期刊论文
| 作者 | Zhang, Weibing1; Sun, Xudong1; Dong, Jing2; Li, Jinan2; Ye, Mingliang2; Zhang, Lingyi1; Ou, Junjie2 |
| 刊名 | RSC ADVANCES
 |
| 出版日期 | 2016 |
| 卷号 | 6期号:114页码:113058-113065 |
| 英文摘要 | The development of hydrophilic stationary phase for highly efficient and selective enrichment and identification of low-abundance glycopeptides from a complex biological sample is an important prerequisite in glycoproteomics research. Herein, a hydrophilic cysteine functionalized macroporous adsorption resin/gold nanoparticle (MAR/Au-Cys) was synthesized and applied to the highly selective enrichment and identification of N-linked glycopeptides. The unique porous structure and high specific surface area of MAR provide abundant bonding sites for the immobilization of Au nanoparticles, and subsequently for the loading of cysteine via robust Au-S bonds. The structure and chemical composition of MAR/Au-Cys were confirmed by scanning electron microscopy, nitrogen adsorption-desorption analysis, mercury intrusion porosimetry and Fourier-transform infrared spectroscopy. Besides, the MAR/Au-Cys exhibited good performance in glycopeptide enrichment of tryptic digests from human immunoglobulin G and horseradish peroxidase by operation of a HILIC-SPE tip, revealing good universality, high enrichment selectivity, low detection limit (2.5 fmol) and satisfactory enrichment recovery (above 79%). Furthermore, the proposed material was successfully utilized in the N-glycoproteomics analysis of a complex biological sample. 1305 unique glycopeptides with 883 N-glycosylation sites from 476 different N-linked glycoproteins were identified from the proteins extracted from mouse liver. These results show that the proposed Au-cysteine modified macroporous adsorption resin is a promising affinity material for glycoproteomics research of real complex biological samples. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Multidisciplinary |
| 关键词[WOS] | HYDROPHILIC INTERACTION CHROMATOGRAPHY ; FUNCTIONALIZED MAGNETIC NANOPARTICLES ; MASS-SPECTROMETRY ; EFFICIENT ENRICHMENT ; GLYCOPROTEINS ; GLYCOSYLATION ; SEPARATION ; HETEROGENEITY ; PURIFICATION ; REBAUDIOSIDE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000389905300039 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/150426]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Weibing,Sun, Xudong,Dong, Jing,et al. Au-cysteine modified macroporous adsorption resin: preparation and highly selective enrichment and identification of N-linked glycopeptides from the complex biological sample[J]. RSC ADVANCES,2016,6(114):113058-113065. |
| APA | Zhang, Weibing.,Sun, Xudong.,Dong, Jing.,Li, Jinan.,Ye, Mingliang.,...&Ou, Junjie.(2016).Au-cysteine modified macroporous adsorption resin: preparation and highly selective enrichment and identification of N-linked glycopeptides from the complex biological sample.RSC ADVANCES,6(114),113058-113065. |
| MLA | Zhang, Weibing,et al."Au-cysteine modified macroporous adsorption resin: preparation and highly selective enrichment and identification of N-linked glycopeptides from the complex biological sample".RSC ADVANCES 6.114(2016):113058-113065. |
入库方式： OAI收割
来源：大连化学物理研究所
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