Probing the Lysine Proximal Microenvironments within Membrane Protein Complexes by Active Dimethyl Labeling and Mass Spectrometry
文献类型：期刊论文
| 作者 | Han, Guangye3; Wang, Fangjun1; Shen, Jian-Ren3,5; Tian, Lirong3,4; Chen, Jun2; Chen, Jin1,4; Liu, Zheyi1,4; Yao, Mingdong2; Wu, Yue1,6; Zhou, Ye1,4 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2016-12-20 |
| 卷号 | 88期号:24页码:12060-12065 |
| 英文摘要 | Positively charged lysines are crucial to maintaining the native structures of proteins and protein complexes by forming hydrogen bonds and electrostatic interactions with their proximal amino acid residues. However, it is still a challenge to develop an efficient method for probing the active proximal microenvironments of lysines without changing their biochemical/physical properties. Herein, we developed an active covalent labeling strategy combined with mass spectrometry to systematically probe the lysine proximal microenvironments within membrane protein complexes (similar to 700 kDa) with high throughput. Our labeling strategy has the advantages of high labeling efficiency and stability, preservation of the active charge states, as well as biological activity of the labeled proteins. In total, 121 lysines with different labeling levels were obtained for the photosystem II complexes from cyanobacteria, red algae, and spinach and provided important insights for understanding the conserved and nonconserved local structures of PSII complexes among evolutionarily divergent species that perform photosynthesis. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | EVOLVING PHOTOSYSTEM-II ; CROSS-LINKED PEPTIDES ; STRUCTURAL-CHARACTERIZATION ; ELECTROSTATIC INTERACTION ; REDUCTIVE METHYLATION ; 33-KDA PROTEIN ; IDENTIFICATION ; CRYSTALLIZATION ; RESIDUES ; DYNAMICS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000390621000015 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/151819]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Energy Dalian Inst Chem Phys, Dalian Natl Lab Clean Energy, State Key Lab Catalysis, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Okayama Univ, Grad Sch Nat Sci & Technol, Photosynth Res Ctr, 1-1,Naka 3 Chome, Okayama 7008530, Japan 6.Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA |
| 推荐引用方式 GB/T 7714 | Han, Guangye,Wang, Fangjun,Shen, Jian-Ren,et al. Probing the Lysine Proximal Microenvironments within Membrane Protein Complexes by Active Dimethyl Labeling and Mass Spectrometry[J]. ANALYTICAL CHEMISTRY,2016,88(24):12060-12065. |
| APA | Han, Guangye.,Wang, Fangjun.,Shen, Jian-Ren.,Tian, Lirong.,Chen, Jun.,...&Zhou, Ye.(2016).Probing the Lysine Proximal Microenvironments within Membrane Protein Complexes by Active Dimethyl Labeling and Mass Spectrometry.ANALYTICAL CHEMISTRY,88(24),12060-12065. |
| MLA | Han, Guangye,et al."Probing the Lysine Proximal Microenvironments within Membrane Protein Complexes by Active Dimethyl Labeling and Mass Spectrometry".ANALYTICAL CHEMISTRY 88.24(2016):12060-12065. |
入库方式： OAI收割
来源：大连化学物理研究所
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