Determination of Glycoproteins by a Self-Assembled 4-Mercaptophenylboronic Acid Film on a Quartz Crystal Microbalance
文献类型：期刊论文
| 作者 | He, Xi-Wen2; Zhang, Yu-Kui2,5; Ma, Xiao-Tong2; Li, Wen-You1,2,3,4 |
| 刊名 | ANALYTICAL SCIENCES
 |
| 出版日期 | 2016-12-01 |
| 卷号 | 32期号:12页码:1277-1282 |
| 关键词 | Quartz crystal microbalance glycoproteins 4-mercaptophenylboronic acid |
| 英文摘要 | Glycosylation plays an important part in many biological processes. However, many glycoproteins are either of low abundance or covered by other components in biological samples. Hence, developing new methods to measure the glycoproteins with both high efficiency and low detection limit is important. In this work, a self-assembled 4-mercaptophenylboronic acid film was coated on a quartz crystal microbalance chip. By optimizing the reaction time and the concentration of 4-mercaptophenylboronic acid, a sensor that specifically responded to glycoproteins was created. Then, several parameters for the prepared sensor were investigated and the working curve for representative glycoprotein-transferrin was established. The linearity range was from 50 to 400 ng/mL and the detection limit was 21.0 ng/mL. The sensor was used to detect transferrin in artificial urine samples. This sensor has a low detection limit of glycoproteins requiring only a small amount of samples, and thus has potential applications in both pharmaceutical and medical areas. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | DISTILLATION-PRECIPITATION POLYMERIZATION ; FUNCTIONALIZED MESOPOROUS SILICA ; SURFACE-PLASMON RESONANCE ; MASS-SPECTROMETRY ; AFFINITY-CHROMATOGRAPHY ; MAGNETIC NANOPARTICLES ; MONOLITHIC COLUMN ; CLICK CHEMISTRY ; ENRICHMENT ; GLYCOPEPTIDES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000390975100002 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/151855]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China 2.Nankai Univ, Coll Chem, Res Ctr Analyt Sci, Tianjin 300071, Peoples R China 3.Tianjin Key Lab Biosensing & Mol Recognit, Tianjin 300071, Peoples R China 4.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China 5.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Xi-Wen,Zhang, Yu-Kui,Ma, Xiao-Tong,et al. Determination of Glycoproteins by a Self-Assembled 4-Mercaptophenylboronic Acid Film on a Quartz Crystal Microbalance[J]. ANALYTICAL SCIENCES,2016,32(12):1277-1282. |
| APA | He, Xi-Wen,Zhang, Yu-Kui,Ma, Xiao-Tong,&Li, Wen-You.(2016).Determination of Glycoproteins by a Self-Assembled 4-Mercaptophenylboronic Acid Film on a Quartz Crystal Microbalance.ANALYTICAL SCIENCES,32(12),1277-1282. |
| MLA | He, Xi-Wen,et al."Determination of Glycoproteins by a Self-Assembled 4-Mercaptophenylboronic Acid Film on a Quartz Crystal Microbalance".ANALYTICAL SCIENCES 32.12(2016):1277-1282. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。