Immobilization-free DNA-based homogeneous electrochemical biosensors
文献类型：期刊论文
| 作者 | Cai, Liang-Yuan; Zhang, Xin-Xiang; Zhang, Fang-Ting; Zhou, Ying-Lin |
| 刊名 | TRAC-TRENDS IN ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2016-12-01 |
| 卷号 | 85页码:17-32 |
| 关键词 | DNA Biosensors Immobilization-free Homogeneous Electrochemistry |
| 英文摘要 | DNA is an excellent biomaterial for building biosensors utilizing diverse DNA interactions with a broad range of biomolecules and chemical compounds. DNA-based electrochemical biosensors have been successfully used in numerous applications with advantages of rapid detection, high sensitivity and low cost. Typical DNA-based electrochemical biosensors require an immobilization step of DNA probe as the biological recognition element, which always suffers from time-consuming and labor-intensive electrode pretreatment and immobilization process, expensive chemical modification, lower reactivity, and complicated operation. Immobilization-free DNA-based electrochemical biosensors can provide powerful complementary tools and novel design strategies for traditional electrochemical DNA biosensors. They would be advantageous in some ways, such as low cost, easy operation, and time saving. More importantly, the homogeneous reaction could increase the reliability and reproducibility of electrochemical sensor without the immobilization step. This review introduces multiple immobilization-free strategies for developing DNA-based homogeneous electrochemical biosensors, aiming at compensating for the deficiencies of heterogeneous modes. The main signal readout modes and signal enhancement strategies will be discussed separately. Additionally, the burgeoning applications combined with miniaturization of electrochemical devices will be highlighted. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | ROLLING-CIRCLE AMPLIFICATION ; HEMIN/G-QUADRUPLEX DNAZYME ; SINGLE-STRANDED-DNA ; TARGET RECYCLING AMPLIFICATION ; HYBRIDIZATION CHAIN-REACTION ; SEQUENCE-SPECIFIC DETECTION ; STRUCTURE-SWITCHING DNA ; REDUCED GRAPHENE OXIDE ; LABEL-FREE ; NUCLEIC-ACID |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000390737700002 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/151856]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Peking Univ, Key Lab Bioorgan Chem & Mol Engn, BNLMS, Minist Educ,Coll Chem, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cai, Liang-Yuan,Zhang, Xin-Xiang,Zhang, Fang-Ting,et al. Immobilization-free DNA-based homogeneous electrochemical biosensors[J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY,2016,85:17-32. |
| APA | Cai, Liang-Yuan,Zhang, Xin-Xiang,Zhang, Fang-Ting,&Zhou, Ying-Lin.(2016).Immobilization-free DNA-based homogeneous electrochemical biosensors.TRAC-TRENDS IN ANALYTICAL CHEMISTRY,85,17-32. |
| MLA | Cai, Liang-Yuan,et al."Immobilization-free DNA-based homogeneous electrochemical biosensors".TRAC-TRENDS IN ANALYTICAL CHEMISTRY 85(2016):17-32. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。