Exploratory Purification of Compounds from Aqueous Extracts of Pinus massoniana Lamb. by Two-Dimensional Preparative Liquid Chromatography
文献类型：期刊论文
| 作者 | Hao, Xiaofeng3; Liang, Xinmiao1; Yang, Fan2; Zhao, Manxi3; Zhang, Xiuli1; Li, Qiang3; Xiao, Yuansheng1; Ke, Xiao3; He, Jian1; Ye, Liang3 |
| 刊名 | CHROMATOGRAPHIA
 |
| 出版日期 | 2017 |
| 卷号 | 80期号:1页码:39-43 |
| 关键词 | Two-dimensional preparative LC Orthogonal RP x HILIC RP x RP Separation strategy |
| 英文摘要 | An off-line orthogonal two-dimensional preparative liquid chromatography method was developed for isolation of high-purity compounds from aqueous extracts of Pinus massoniana Lamb., which has efficient therapeutic properties in recipes of traditional Chinese medicines. A polar-enhanced reversed-phase (RP) column, XAqua C18 was selected for the first-dimensional separation. A HILIC column and a hydrophobic RP column were both explored in the second dimension. The preparation results indicated hydrophilic RP x HILIC mode would provide better separation efficiency for polar complexes, i.e., the second-dimensional separation was finished in less than 30 min and high-purity compounds were obtained. In this work, 16 fractions were collected efficiently in the first dimension with a recovery up to 96%, and six compounds with high purity and enough quantity were identified by NMR and high resolution mass spectrometry, including epicatechin, betuloside, taxifolin-3-O-xyloside, cedrusin-4-O-glucoside, massonianoside C and massonianoside D, and five are glycosides, including flavonoid glycosides, lignanoid glycosides and an arylbutanoid glycoside. Betuloside was first reported in the preparation of Pinus massoniana Lamb. This work demonstrated a flexible strategy of compromise in between time and resolution for selecting solvent and column to enhance preparative efficiency of polar complex systems. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | FLAVONOID GLYCOSIDES ; STATIONARY PHASES ; IDENTIFICATION ; NEEDLES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000392315400006 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/151872]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Analyt Chem Serv Ctr, Dalian 116023, Peoples R China 3.Chengdu Kanghong Pharmaceut Ltd Co, Chengdu 610036, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hao, Xiaofeng,Liang, Xinmiao,Yang, Fan,et al. Exploratory Purification of Compounds from Aqueous Extracts of Pinus massoniana Lamb. by Two-Dimensional Preparative Liquid Chromatography[J]. CHROMATOGRAPHIA,2017,80(1):39-43. |
| APA | Hao, Xiaofeng.,Liang, Xinmiao.,Yang, Fan.,Zhao, Manxi.,Zhang, Xiuli.,...&Ke, Zunhong.(2017).Exploratory Purification of Compounds from Aqueous Extracts of Pinus massoniana Lamb. by Two-Dimensional Preparative Liquid Chromatography.CHROMATOGRAPHIA,80(1),39-43. |
| MLA | Hao, Xiaofeng,et al."Exploratory Purification of Compounds from Aqueous Extracts of Pinus massoniana Lamb. by Two-Dimensional Preparative Liquid Chromatography".CHROMATOGRAPHIA 80.1(2017):39-43. |
入库方式： OAI收割
来源：大连化学物理研究所
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