Probing the Binding Interfaces of Histone-Aptamer by Photo Cross-Linking Mass Spectrometry
文献类型：期刊论文
| 作者 | Zhang, Yukui1,4; He, Xiwen1; Lu, Congcong1; Tian, Shanshan2; Zhai, Guijin2; Yuan, Zuofei3; Li, Yijun1; Zhang, Kai1,2 |
| 刊名 | ACS CHEMICAL BIOLOGY
 |
| 出版日期 | 2017 |
| 卷号 | 12期号:1页码:57-62 |
| 英文摘要 | Histone proteins, which could interact with DNA, play important roles in the regulation of chromatin structures, transcription, and other DNA-based biological processes. Here, we developed a novel aptamer-based probe for the analysis of histone H4-aptamer interfaces. This probe contains a DNA sequence for specific recognition of histone H4, a biotin tag for affinity enrichment, an aryl azide photoactive group for cross-linking and a cleavable disulfide group to dissociate aptamer from labeled histones. We successfully achieved specific enrichment of histone H4 and further developed a new analysis strategy for histone-aptamer interaction by photo cross-linking mass spectrometry. The binding area of histone H4 to aptamer was investigated and discussed for the first time. This strategy exhibits great potential and Might further contribute to the understanding of histone-DNA interaction patterns. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | INDUCED STRUCTURAL-CHANGES ; PROTEIN INTERACTIONS ; DNA APTAMER ; STREPTAVIDIN ; ASSEMBLIES ; MOLECULE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000392569400007 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/151884]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China 2.Tianjin Med Univ, Dept Biochem & Mol Biol, Tianjin Key Lab Med Epigenet, Key Lab Immune Microenvironm & Dis,Ministry Educ, Tianjin 300070, Peoples R China 3.Univ Penn, Perelman Sch Med, Dept Biochem & Biophys, Philadelphia, PA 19104 USA 4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yukui,He, Xiwen,Lu, Congcong,et al. Probing the Binding Interfaces of Histone-Aptamer by Photo Cross-Linking Mass Spectrometry[J]. ACS CHEMICAL BIOLOGY,2017,12(1):57-62. |
| APA | Zhang, Yukui.,He, Xiwen.,Lu, Congcong.,Tian, Shanshan.,Zhai, Guijin.,...&Zhang, Kai.(2017).Probing the Binding Interfaces of Histone-Aptamer by Photo Cross-Linking Mass Spectrometry.ACS CHEMICAL BIOLOGY,12(1),57-62. |
| MLA | Zhang, Yukui,et al."Probing the Binding Interfaces of Histone-Aptamer by Photo Cross-Linking Mass Spectrometry".ACS CHEMICAL BIOLOGY 12.1(2017):57-62. |
入库方式： OAI收割
来源：大连化学物理研究所
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