Ultrathin Au nanowires assisted magnetic graphene-silica ZIC-HILIC composites for highly specific enrichment of N-linked glycopeptides
文献类型：期刊论文
| 作者 | Zhang, Yangjun1; Zhang, Yukui2; Qian, Xiaohong1; Jiao, Fenglong1,3; Gao, Fangyuan1; Wang, Heping1; Deng, Yulin3 |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2017-06-01 |
| 卷号 | 970页码:47-56 |
| 关键词 | Au nanowires Graphene oxide L-Cys Glycopeptides Enrichment |
| 英文摘要 | Protein glycosylation has been proven to participate in a variety of complex biological processes; however, the low abundance of glycopeptides in natural samples makes it essential to develop methods to isolate and enrich glycopeptides. In this study, a novel ultrathin Au nanowire assisted zwitterionic hydrophilic magnetic graphene oxide ( GO-Fe3O4/SiO2/AuNWs/L-Cys) was synthesized with the good biocompatibility of GO, strong magnetic responses of Fe3O4, large surface area of ultrathin Au nanowires and excellent hydrophilicity of L-Cys via four simple and rapid steps. The ultrathin Au nanowires have a one-dimensional structure and were easily grafted with an abundant amount of L-Cys for the enrichment of glycopeptides. After the GO-Fe3O4/SiO2/AuNWs/L-Cys composites were applied to glycopeptide enrichment, 26 glycopeptides from a human IgG digest could be identified, with a detection limit as low as 10 fmol. Due to the abundant amount of grafted L-Cys, the composites also showed a large binding capacity ( 150 mu g mg(-1)). Furthermore, the composites were applied for the analysis of real biological samples. A total of 793 glycopeptides from 467 glycoproteins were identified in three replicate analyses of 40 mg of mouse liver proteins. The results demonstrated the great potential of GO-Fe3O4/SiO2/AuNWs/L-Cys composites for the analysis of glycoproteins. (C) 2017 Elsevier B.V. All rightd reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | SELECTIVE ENRICHMENT ; MASS-SPECTROMETRY ; FACILE SYNTHESIS ; OXIDE ; NANOPARTICLES ; GLYCOPROTEINS ; EFFICIENT ; GLYCOPROTEINS/GLYCOPEPTIDES ; NANOCOMPOSITES ; IDENTIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000399781700005 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/151978]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Natl Ctr Prot Sci, State Key Lab Prote, Beijing 102200, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China 3.Beijing Inst Technol, Sch Life Sci & Technol, Beijing 100081, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yangjun,Zhang, Yukui,Qian, Xiaohong,et al. Ultrathin Au nanowires assisted magnetic graphene-silica ZIC-HILIC composites for highly specific enrichment of N-linked glycopeptides[J]. ANALYTICA CHIMICA ACTA,2017,970:47-56. |
| APA | Zhang, Yangjun.,Zhang, Yukui.,Qian, Xiaohong.,Jiao, Fenglong.,Gao, Fangyuan.,...&Deng, Yulin.(2017).Ultrathin Au nanowires assisted magnetic graphene-silica ZIC-HILIC composites for highly specific enrichment of N-linked glycopeptides.ANALYTICA CHIMICA ACTA,970,47-56. |
| MLA | Zhang, Yangjun,et al."Ultrathin Au nanowires assisted magnetic graphene-silica ZIC-HILIC composites for highly specific enrichment of N-linked glycopeptides".ANALYTICA CHIMICA ACTA 970(2017):47-56. |
入库方式： OAI收割
来源：大连化学物理研究所
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