Site-directed immobilization antibody for alpha-fetoprotein detection by optical biosensor
文献类型：期刊论文
| 作者 | Huang, Chenghong; Yang, QingLin; Song, Fayi; Chen, Nan; Liao, Xiaoling; Yao, Bo; Zhang, ShiTong; Chen, Yanyan; Jin G(靳刚) ; Huang, CH (reprint author), Chongqing Univ Sci & Technol, Chongqing, Peoples R China.
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| 刊名 | INTEGRATED FERROELECTRICS
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| 出版日期 | 2016 |
| 卷号 | 171期号:1页码:70-78 |
| 关键词 | Alpha-fetoprotein Imaging Ellipsometry Immunoassay |
| ISSN号 | 1058-4587 |
| DOI | 10.1080/10584587.2016.1172004 |
| 文献子类 | Article |
| 英文摘要 | Carbohydrate side chain in antibody Fc region can be oxidized into aldehyde group by sodium m-periodate, which can simultaneously couple with the amino-group on silicon surface obtained by chemistry modification. This combination will introduce C=N linkage and site-directed immobilization of antibody, which was confirmed by AFM observation. The acquired antibody layer can then be used for immunoassay for alpha-fetoprotein (AFP). The combined antigen is directly detected by imaging ellipsometry in grayscale format. The proposed method was further compared with commercial kit by determination 20 clinic samples with significant correlation (R = 0.93). |
| 分类号 | Q4 |
| WOS关键词 | ATOMIC-FORCE MICROSCOPY ; IMAGING ELLIPSOMETRY ; VISUALIZATION |
| WOS研究方向 | Engineering, Electrical & Electronic ; Physics, Applied ; Physics, Condensed Matter |
| 语种 | 英语 |
| WOS记录号 | WOS:000378406600010 |
| 资助机构 | This work is supported by the cooperative project of academician workstation of Chongqing University of Science & Technology (CKYS2014Y5), Research Foundation of Chongqing University of Science & Technology (CK2014B4) and Chongqing pogram of Application Foundation and Advanced Technology(cstc2014jcyjA50032, cstc2014jcyjA10029). |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/71955]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 通讯作者 | Huang, CH (reprint author), Chongqing Univ Sci & Technol, Chongqing, Peoples R China.; Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou, Peoples R China.; Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Beijing, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Huang, Chenghong,Yang, QingLin,Song, Fayi,et al. Site-directed immobilization antibody for alpha-fetoprotein detection by optical biosensor[J]. INTEGRATED FERROELECTRICS,2016,171(1):70-78. |
| APA | Huang, Chenghong.,Yang, QingLin.,Song, Fayi.,Chen, Nan.,Liao, Xiaoling.,...&Jin, G .(2016).Site-directed immobilization antibody for alpha-fetoprotein detection by optical biosensor.INTEGRATED FERROELECTRICS,171(1),70-78. |
| MLA | Huang, Chenghong,et al."Site-directed immobilization antibody for alpha-fetoprotein detection by optical biosensor".INTEGRATED FERROELECTRICS 171.1(2016):70-78. |
入库方式： OAI收割
来源：力学研究所
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