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In vitro antimicrobial effects and mechanism of atmospheric-pressure He/O-2 plasma jet on Staphylococcus aureus biofilm

文献类型：期刊论文

作者Xu, Zimu1; Shen, Jie2; Cheng, Cheng2,3; Hu, Shuheng1; Lan, Yan2; Chu, Paul K.4
刊名JOURNAL OF PHYSICS D-APPLIED PHYSICS
出版日期2017-03-15
卷号50期号:10
关键词Atmospheric-pressure Plasma Jet (Appj) Staphylococcus Aureus Biofilm Inactivation Reactive Oxygen Species (Ros)
DOI10.1088/1361-6463/aa593f
文献子类Article
英文摘要The antimicrobial effects and associated mechanism of inactivation of Staphylococcus aureus (S. aureus) NCTC-8325 biofilms induced by a He/O-2 atmospheric-pressure plasma jet (APPJ) are investigated in vitro. According to CFU (colony forming units) counting and the resazurin-based assay, the 10 min He/O-2 (0.5%) APPJ treatment produces the optimal inactivation efficacy (> 5 log10 ml(-1)) against the S. aureus biofilm and 5% of the bacteria enter a viable but non-culturable (VBNC) state. Meanwhile, 94% of the bacteria suffer from membrane damage according to SYTO 9/PI counterstaining. Scanning electron microscopy (SEM) reveals that plasma exposure erodes the extracellular polymeric substances (EPS) and then the cellular structure. The H2DCFDA-stained biofilms show larger concentrations of intracellular reactive oxygen species (ROS) in membrane-intact bacteria with increasing plasma dose. The admixture of oxygen in the working gas highly contributes to the deactivation efficacy of the APPJ against S. aureus and the plasma-induced endogenous ROS may work together with the discharge-generated ROS to continuously damage the bacterial membrane structure leading to deactivation of the biofilm microbes.
WOS关键词NONTHERMAL PLASMA ; BACTERIAL BIOFILMS ; PSEUDOMONAS-AERUGINOSA ; DISCHARGE PLASMA ; INFECTIONS ; EFFICACY ; STERILIZATION ; CHLORHEXIDINE ; DISINFECTION ; INACTIVATION
WOS研究方向Physics
语种英语
WOS记录号WOS:000401725300001
资助机构National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; National Natural Science Foundation of China(51541807) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences(DSJJ-14-YY02) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; City University of Hong Kong Strategic Research Grant (SRG)(7004188) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215) ; Hong Kong Research Grants Council (RGC) General Research Funds (GRF)(CityU 11301215)
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/33414]  
专题合肥物质科学研究院_中科院等离子体物理研究所
作者单位1.Hefei Univ Technol, Sch Resources & Environm Engn, Hefei 230009, Anhui, Peoples R China
2.Chinese Acad Sci, Inst Plasma Phys, POB 1126, Hefei 230031, Peoples R China
3.Chinese Acad Sci, Hefei Inst Phys Sci, Ctr Med Phys & Technol, Hefei 230031, Peoples R China
4.City Univ Hong Kong, Dept Phys & Mat Sci, Tat Chee Ave, Kowloon, Hong Kong, Peoples R China
推荐引用方式
GB/T 7714		 Xu, Zimu,Shen, Jie,Cheng, Cheng,et al. In vitro antimicrobial effects and mechanism of atmospheric-pressure He/O-2 plasma jet on Staphylococcus aureus biofilm[J]. JOURNAL OF PHYSICS D-APPLIED PHYSICS,2017,50(10).
APA Xu, Zimu,Shen, Jie,Cheng, Cheng,Hu, Shuheng,Lan, Yan,&Chu, Paul K..(2017).In vitro antimicrobial effects and mechanism of atmospheric-pressure He/O-2 plasma jet on Staphylococcus aureus biofilm.JOURNAL OF PHYSICS D-APPLIED PHYSICS,50(10).
MLA Xu, Zimu,et al."In vitro antimicrobial effects and mechanism of atmospheric-pressure He/O-2 plasma jet on Staphylococcus aureus biofilm".JOURNAL OF PHYSICS D-APPLIED PHYSICS 50.10(2017).

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来源：合肥物质科学研究院

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