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Calix[4]arene crown ether as an oriented linker for highly sensitive detection of zinc ions using a peptide probe

文献类型：期刊论文

作者Jia, Shengsong1,2; Shao, Min3; Zou, Fei2; Wu, Beiping4; Zhou, Hongjian5; Chen, Hongxia1,2
刊名ANALYTICAL METHODS
出版日期2016
卷号8期号:19页码:3959-3965
DOI10.1039/c6ay00642f
文献子类Article
英文摘要Peptides, as the simplest biological recognition elements have been developed as probes to capture multitudinous targets. One important factor in fabricating peptide biochips is to immobilize peptides without losing their activity on a solid phase. To keep them functional, it is necessary to immobilize peptide in an oriented way. Calix[4]arene crown ether (CC) has been used as a protein linker molecule on the solid surface. In this study, calix[4]arene crown ether was self-assembled and modified on a gold surface. The calix[4]arene crown ether monolayer was characterized by EIS and SPR. An elaborately designed peptide probe Ac-CCPGCAAAARRRR-NH2 is employed here for the assay of Zn2+. This tridecapeptide consists of a binding part (CCPGC), spacer (AAAA) and immobilization part (RRRR) to interact with a CC SAM. We compared the sensitivity and the specificity of the linker molecules with those of common attachment agents using a zinc ion binding peptide. The fabricated chip showed a superior sensitivity and a much lower detection limit than those chips prepared by other methods. Thus, the calix[4]arene crown ether chip can be used as a powerful peptide linker with a wide range of applications, including peptide-drug interaction, peptide-cell interaction, and an enzyme activity assay.
WOS关键词SURFACE-PLASMON RESONANCE ; ON FLUORESCENT-PROBE ; SELECTIVE DETECTION ; PROTEIN IMMOBILIZATION ; DERIVATIVE MONOLAYERS ; SENSOR ; RECOGNITION ; FABRICATION ; ANTIBODY ; BINDING
WOS研究方向Chemistry ; Food Science & Technology ; Spectroscopy
语种英语
WOS记录号WOS:000376043300013
资助机构National Natural Science Foundation of China(61275085 ; National Natural Science Foundation of China(61275085 ; National Natural Science Foundation of China(61275085 ; National Natural Science Foundation of China(61275085 ; National Natural Science Foundation of China(61275085 ; National Natural Science Foundation of China(61275085 ; National Natural Science Foundation of China(61275085 ; National Natural Science Foundation of China(61275085 ; 31100560) ; 31100560) ; 31100560) ; 31100560) ; 31100560) ; 31100560) ; 31100560) ; 31100560) ; National Natural Science Foundation of China(61275085 ; National Natural Science Foundation of China(61275085 ; National Natural Science Foundation of China(61275085 ; National Natural Science Foundation of China(61275085 ; National Natural Science Foundation of China(61275085 ; National Natural Science Foundation of China(61275085 ; National Natural Science Foundation of China(61275085 ; National Natural Science Foundation of China(61275085 ; 31100560) ; 31100560) ; 31100560) ; 31100560) ; 31100560) ; 31100560) ; 31100560) ; 31100560)
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/22018]  
专题合肥物质科学研究院_中科院固体物理研究所
作者单位1.Bright Dairy & Food Co Ltd, State Key Lab Dairy Biotechnol, Shanghai 200444, Peoples R China
2.Shanghai Univ, Sch Life Sci, Lab Biosensing Technol, Shanghai 200444, Peoples R China
3.Shanghai Univ, Sch Mat Sci & Engn, Instrumental Anal & Res Ctr, Shanghai 200444, Peoples R China
4.Shanghai Univ, Sch Life Sci, Shanghai Key Lab Bioenergy Crops, Shanghai 200444, Peoples R China
5.Chinese Acad Sci, Inst Solid State Phys, Anhui Key Lab Nanomat & Nanotechnol, Ctr Environm & Energy Nanomat,Key Lab Mat Phys, Hefei 230031, Peoples R China
推荐引用方式
GB/T 7714		 Jia, Shengsong,Shao, Min,Zou, Fei,et al. Calix[4]arene crown ether as an oriented linker for highly sensitive detection of zinc ions using a peptide probe[J]. ANALYTICAL METHODS,2016,8(19):3959-3965.
APA Jia, Shengsong,Shao, Min,Zou, Fei,Wu, Beiping,Zhou, Hongjian,&Chen, Hongxia.(2016).Calix[4]arene crown ether as an oriented linker for highly sensitive detection of zinc ions using a peptide probe.ANALYTICAL METHODS,8(19),3959-3965.
MLA Jia, Shengsong,et al."Calix[4]arene crown ether as an oriented linker for highly sensitive detection of zinc ions using a peptide probe".ANALYTICAL METHODS 8.19(2016):3959-3965.

入库方式： OAI收割

来源：合肥物质科学研究院

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