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Diaminodiacid Bridges to Improve Folding and Tune the Bioactivity of Disulfide-Rich Peptides

文献类型：期刊论文

作者Guo, Ye1; Sun, De-Meng1; Wang, Feng-Liang2; He, Yao3,4,5; Liu, Lei1; Tian, Chang-Lin3,4,5
刊名ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
出版日期2015-11-23
卷号54期号:48页码:14276-14281
关键词Disulfide-rich Peptide Peptide Therapeutics Peptide Synthesis Protein Engineering Protein Folding
DOI10.1002/anie.201500699
文献子类Article
英文摘要Disulfide-rich peptides containing three or more disulfide bonds are promising therapeutic and diagnostic agents, but their preparation is often limited by the tedious and low-yielding folding process. We found that a single cystine-to-diaminodiacid replacement could significantly increase the folding efficiency of disulfide-rich peptides and thus improve their production yields. The practicality of this strategy was demonstrated by the synthesis and folding of derivatives of the mu-conotoxin SIIIA, the preclinical hormone hepcidin, and the trypsin inhibitor EETI-II. NMR and X-ray crystallography studies confirmed that these derivatives of disulfide-rich peptide retained the correct three-dimensional conformations. Moreover, the cystine-to-diaminodiacid replacement enabled structural tuning, thereby leading to an EETI-II derivative with higher bioactivity than the native peptide.
WOS关键词ALPHA-CONOTOXIN ; CYSTINE-KNOT ; DISELENIDE BRIDGES ; CHEMICAL-SYNTHESIS ; DICARBA ANALOGS ; SODIUM-CHANNELS ; EETI-II ; HEPCIDIN ; STABILITY ; DISCOVERY
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000367724000008
资助机构"863" Program of the Ministry of Science and Technology(2012AA02A700) ; "863" Program of the Ministry of Science and Technology(2012AA02A700) ; "863" Program of the Ministry of Science and Technology(2012AA02A700) ; "863" Program of the Ministry of Science and Technology(2012AA02A700) ; "863" Program of the Ministry of Science and Technology(2012AA02A700) ; "863" Program of the Ministry of Science and Technology(2012AA02A700) ; "863" Program of the Ministry of Science and Technology(2012AA02A700) ; "863" Program of the Ministry of Science and Technology(2012AA02A700) ; National Basic Research Program of China (973 program)(2013CB932800 ; National Basic Research Program of China (973 program)(2013CB932800 ; National Basic Research Program of China (973 program)(2013CB932800 ; National Basic Research Program of China (973 program)(2013CB932800 ; National Basic Research Program of China (973 program)(2013CB932800 ; National Basic Research Program of China (973 program)(2013CB932800 ; National Basic Research Program of China (973 program)(2013CB932800 ; National Basic Research Program of China (973 program)(2013CB932800 ; NSFC(91313301 ; NSFC(91313301 ; NSFC(91313301 ; NSFC(91313301 ; NSFC(91313301 ; NSFC(91313301 ; NSFC(91313301 ; NSFC(91313301 ; Specialized Research Fund for the Doctoral Program of Higher Education(20120002130004) ; Specialized Research Fund for the Doctoral Program of Higher Education(20120002130004) ; Specialized Research Fund for the Doctoral Program of Higher Education(20120002130004) ; Specialized Research Fund for the Doctoral Program of Higher Education(20120002130004) ; Specialized Research Fund for the Doctoral Program of Higher Education(20120002130004) ; Specialized Research Fund for the Doctoral Program of Higher Education(20120002130004) ; Specialized Research Fund for the Doctoral Program of Higher Education(20120002130004) ; Specialized Research Fund for the Doctoral Program of Higher Education(20120002130004) ; 2015CB910100) ; 2015CB910100) ; 2015CB910100) ; 2015CB910100) ; 2015CB910100) ; 2015CB910100) ; 2015CB910100) ; 2015CB910100) ; 21225207) ; 21225207) ; 21225207) ; 21225207) ; 21225207) ; 21225207) ; 21225207) ; 21225207) ; "863" Program of the Ministry of Science and Technology(2012AA02A700) ; "863" Program of the Ministry of Science and Technology(2012AA02A700) ; "863" Program of the Ministry of Science and Technology(2012AA02A700) ; "863" Program of the Ministry of Science and Technology(2012AA02A700) ; "863" Program of the Ministry of Science and Technology(2012AA02A700) ; "863" Program of the Ministry of Science and Technology(2012AA02A700) ; "863" Program of the Ministry of Science and Technology(2012AA02A700) ; "863" Program of the Ministry of Science and Technology(2012AA02A700) ; National Basic Research Program of China (973 program)(2013CB932800 ; National Basic Research Program of China (973 program)(2013CB932800 ; National Basic Research Program of China (973 program)(2013CB932800 ; National Basic Research Program of China (973 program)(2013CB932800 ; National Basic Research Program of China (973 program)(2013CB932800 ; National Basic Research Program of China (973 program)(2013CB932800 ; National Basic Research Program of China (973 program)(2013CB932800 ; National Basic Research Program of China (973 program)(2013CB932800 ; NSFC(91313301 ; NSFC(91313301 ; NSFC(91313301 ; NSFC(91313301 ; NSFC(91313301 ; NSFC(91313301 ; NSFC(91313301 ; NSFC(91313301 ; Specialized Research Fund for the Doctoral Program of Higher Education(20120002130004) ; Specialized Research Fund for the Doctoral Program of Higher Education(20120002130004) ; Specialized Research Fund for the Doctoral Program of Higher Education(20120002130004) ; Specialized Research Fund for the Doctoral Program of Higher Education(20120002130004) ; Specialized Research Fund for the Doctoral Program of Higher Education(20120002130004) ; Specialized Research Fund for the Doctoral Program of Higher Education(20120002130004) ; Specialized Research Fund for the Doctoral Program of Higher Education(20120002130004) ; Specialized Research Fund for the Doctoral Program of Higher Education(20120002130004) ; 2015CB910100) ; 2015CB910100) ; 2015CB910100) ; 2015CB910100) ; 2015CB910100) ; 2015CB910100) ; 2015CB910100) ; 2015CB910100) ; 21225207) ; 21225207) ; 21225207) ; 21225207) ; 21225207) ; 21225207) ; 21225207) ; 21225207)
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/22229]  
专题合肥物质科学研究院_中科院强磁场科学中心
作者单位1.Tsinghua Univ, Tsinghua Peking Ctr Life Sci, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Minist Educ,Dept Chem, Beijing 100084, Peoples R China
2.Univ Sci & Technol China, Dept Chem, Hefei 230026, Peoples R China
3.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei, Peoples R China
4.Univ Sci & Technol China, Sch Life Sci, Hefei, Peoples R China
5.Chinese Acad Sci, High Field Magnet Lab, Hefei 230027, Peoples R China
推荐引用方式
GB/T 7714		 Guo, Ye,Sun, De-Meng,Wang, Feng-Liang,et al. Diaminodiacid Bridges to Improve Folding and Tune the Bioactivity of Disulfide-Rich Peptides[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2015,54(48):14276-14281.
APA Guo, Ye,Sun, De-Meng,Wang, Feng-Liang,He, Yao,Liu, Lei,&Tian, Chang-Lin.(2015).Diaminodiacid Bridges to Improve Folding and Tune the Bioactivity of Disulfide-Rich Peptides.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,54(48),14276-14281.
MLA Guo, Ye,et al."Diaminodiacid Bridges to Improve Folding and Tune the Bioactivity of Disulfide-Rich Peptides".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 54.48(2015):14276-14281.

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来源：合肥物质科学研究院

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