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  2. 合肥物质科学研究院
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  4. 中科院强磁场科学中心
Structural basis for receptor recognition and pore formation of a zebrafish aerolysin-like protein

文献类型：期刊论文

作者Jia, Ning1,2; Liu, Nan3; Cheng, Wang1,2; Jiang, Yong-Liang1,2; Sun, Hui1,2; Chen, Lan-Lan1,2; Peng, Junhui1,2; Zhang, Yonghui1,2; Ding, Yue-He4; Zhang, Zhi-Hui1,2
刊名EMBO REPORTS
出版日期2016-02-01
卷号17期号:2页码:235-248
关键词Crystal Structure Electron Microscopy Reconstruction High-mannose Glycan Pore-forming Protein Vertebrate
文献子类Article
英文摘要Various aerolysin-like pore-forming proteins have been identified from bacteria to vertebrates. However, the mechanism of receptor recognition and/or pore formation of the eukaryotic members remains unknown. Here, we present the first crystal and electron microscopy structures of a vertebrate aerolysin-like protein from Danio rerio, termed Dln1, before and after pore formation. Each subunit of Dln1 dimer comprises a -prism lectin module followed by an aerolysin module. Specific binding of the lectin module toward high-mannose glycans triggers drastic conformational changes of the aerolysin module in a pH-dependent manner, ultimately resulting in the formation of a membrane-bound octameric pore. Structural analyses combined with computational simulations and biochemical assays suggest a pore-forming process with an activation mechanism distinct from the previously characterized bacterial members. Moreover, Dln1 and its homologs are ubiquitously distributed in bony fishes and lamprey, suggesting a novel fish-specific defense molecule.
WOS关键词FORMING TOXIN AEROLYSIN ; CARBOHYDRATE-BINDING ; CROSS-LINKING ; INACTIVATING PROTEIN ; MOLECULAR-DYNAMICS ; CRYSTAL-STRUCTURE ; PLASMA-MEMBRANE ; LECTIN REVEALS ; CDNA CLONING ; CYANOVIRIN-N
WOS研究方向Biochemistry & Molecular Biology ; Cell Biology
语种英语
WOS记录号WOS:000369960600013
资助机构Ministry of Science and Technology of China(2013CB835300 ; Ministry of Science and Technology of China(2013CB835300 ; Ministry of Science and Technology of China(2013CB835300 ; Ministry of Science and Technology of China(2013CB835300 ; Ministry of Science and Technology of China(2013CB835300 ; Ministry of Science and Technology of China(2013CB835300 ; Ministry of Science and Technology of China(2013CB835300 ; Ministry of Science and Technology of China(2013CB835300 ; National Natural Science Foundation of China(31270760) ; National Natural Science Foundation of China(31270760) ; National Natural Science Foundation of China(31270760) ; National Natural Science Foundation of China(31270760) ; National Natural Science Foundation of China(31270760) ; National Natural Science Foundation of China(31270760) ; National Natural Science Foundation of China(31270760) ; National Natural Science Foundation of China(31270760) ; Innovation Center for Cell Signaling Network ; Innovation Center for Cell Signaling Network ; Innovation Center for Cell Signaling Network ; Innovation Center for Cell Signaling Network ; Innovation Center for Cell Signaling Network ; Innovation Center for Cell Signaling Network ; Innovation Center for Cell Signaling Network ; Innovation Center for Cell Signaling Network ; Hefei Science Center of CAS(2015SRG-HSC045 ; Hefei Science Center of CAS(2015SRG-HSC045 ; Hefei Science Center of CAS(2015SRG-HSC045 ; Hefei Science Center of CAS(2015SRG-HSC045 ; Hefei Science Center of CAS(2015SRG-HSC045 ; Hefei Science Center of CAS(2015SRG-HSC045 ; Hefei Science Center of CAS(2015SRG-HSC045 ; Hefei Science Center of CAS(2015SRG-HSC045 ; Program for Changjiang Scholars and Innovative Research Team in University ; Program for Changjiang Scholars and Innovative Research Team in University ; Program for Changjiang Scholars and Innovative Research Team in University ; Program for Changjiang Scholars and Innovative Research Team in University ; Program for Changjiang Scholars and Innovative Research Team in University ; Program for Changjiang Scholars and Innovative Research Team in University ; Program for Changjiang Scholars and Innovative Research Team in University ; Program for Changjiang Scholars and Innovative Research Team in University ; 2015CB910100 ; 2015CB910100 ; 2015CB910100 ; 2015CB910100 ; 2015CB910100 ; 2015CB910100 ; 2015CB910100 ; 2015CB910100 ; 2015SRG-HSC046) ; 2015SRG-HSC046) ; 2015SRG-HSC046) ; 2015SRG-HSC046) ; 2015SRG-HSC046) ; 2015SRG-HSC046) ; 2015SRG-HSC046) ; 2015SRG-HSC046) ; 2014CB910100) ; 2014CB910100) ; 2014CB910100) ; 2014CB910100) ; 2014CB910100) ; 2014CB910100) ; 2014CB910100) ; 2014CB910100) ; Ministry of Science and Technology of China(2013CB835300 ; Ministry of Science and Technology of China(2013CB835300 ; Ministry of Science and Technology of China(2013CB835300 ; Ministry of Science and Technology of China(2013CB835300 ; Ministry of Science and Technology of China(2013CB835300 ; Ministry of Science and Technology of China(2013CB835300 ; Ministry of Science and Technology of China(2013CB835300 ; Ministry of Science and Technology of China(2013CB835300 ; National Natural Science Foundation of China(31270760) ; National Natural Science Foundation of China(31270760) ; National Natural Science Foundation of China(31270760) ; National Natural Science Foundation of China(31270760) ; National Natural Science Foundation of China(31270760) ; National Natural Science Foundation of China(31270760) ; National Natural Science Foundation of China(31270760) ; National Natural Science Foundation of China(31270760) ; Innovation Center for Cell Signaling Network ; Innovation Center for Cell Signaling Network ; Innovation Center for Cell Signaling Network ; Innovation Center for Cell Signaling Network ; Innovation Center for Cell Signaling Network ; Innovation Center for Cell Signaling Network ; Innovation Center for Cell Signaling Network ; Innovation Center for Cell Signaling Network ; Hefei Science Center of CAS(2015SRG-HSC045 ; Hefei Science Center of CAS(2015SRG-HSC045 ; Hefei Science Center of CAS(2015SRG-HSC045 ; Hefei Science Center of CAS(2015SRG-HSC045 ; Hefei Science Center of CAS(2015SRG-HSC045 ; Hefei Science Center of CAS(2015SRG-HSC045 ; Hefei Science Center of CAS(2015SRG-HSC045 ; Hefei Science Center of CAS(2015SRG-HSC045 ; Program for Changjiang Scholars and Innovative Research Team in University ; Program for Changjiang Scholars and Innovative Research Team in University ; Program for Changjiang Scholars and Innovative Research Team in University ; Program for Changjiang Scholars and Innovative Research Team in University ; Program for Changjiang Scholars and Innovative Research Team in University ; Program for Changjiang Scholars and Innovative Research Team in University ; Program for Changjiang Scholars and Innovative Research Team in University ; Program for Changjiang Scholars and Innovative Research Team in University ; 2015CB910100 ; 2015CB910100 ; 2015CB910100 ; 2015CB910100 ; 2015CB910100 ; 2015CB910100 ; 2015CB910100 ; 2015CB910100 ; 2015SRG-HSC046) ; 2015SRG-HSC046) ; 2015SRG-HSC046) ; 2015SRG-HSC046) ; 2015SRG-HSC046) ; 2015SRG-HSC046) ; 2015SRG-HSC046) ; 2015SRG-HSC046) ; 2014CB910100) ; 2014CB910100) ; 2014CB910100) ; 2014CB910100) ; 2014CB910100) ; 2014CB910100) ; 2014CB910100) ; 2014CB910100)
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/22285]  
专题合肥物质科学研究院_中科院强磁场科学中心
作者单位1.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230026, Peoples R China
2.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Peoples R China
3.Tsinghua Univ, Tsinghua Peking Joint Ctr Life Sci, Struct Biol Ctr, Minist Educ,Key Lab Prot Sci,Sch Life Sci, Beijing 100084, Peoples R China
4.Natl Inst Biol Sci, Beijing, Peoples R China
5.Chinese Acad Sci, High Field Magnet Lab, Hefei Inst Phys Sci, Hefei, Peoples R China
6.Univ Alabama Birmingham, Sch Dent, Dept Microbiol, Birmingham, AL 35294 USA
7.Univ Alabama Birmingham, Sch Dent, Dept Pediat Dent, Birmingham, AL 35294 USA
8.Univ Alabama Birmingham, Sch Med, Dept Microbiol, Birmingham, AL 35294 USA
9.Univ Alabama Birmingham, Sch Med, Dept Pediat Dent, Birmingham, AL USA
推荐引用方式
GB/T 7714		 Jia, Ning,Liu, Nan,Cheng, Wang,et al. Structural basis for receptor recognition and pore formation of a zebrafish aerolysin-like protein[J]. EMBO REPORTS,2016,17(2):235-248.
APA Jia, Ning.,Liu, Nan.,Cheng, Wang.,Jiang, Yong-Liang.,Sun, Hui.,...&Zhou, Cong-Zhao.(2016).Structural basis for receptor recognition and pore formation of a zebrafish aerolysin-like protein.EMBO REPORTS,17(2),235-248.
MLA Jia, Ning,et al."Structural basis for receptor recognition and pore formation of a zebrafish aerolysin-like protein".EMBO REPORTS 17.2(2016):235-248.

入库方式： OAI收割

来源：合肥物质科学研究院

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