Total chemical synthesis of photoactivatable proteins for light-controlled manipulation of antigen-antibody interactions
文献类型：期刊论文
| 作者 | Tang, Shan1; Wan, Zhengpeng2; Gao, Yiren2; Zheng, Ji-Shen3; Wang, Jing2; Si, Yan-Yan1; Chen, Xin4; Qi, Hai4; Liu, Lei1 ; Liu, Wanli2
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| 刊名 | CHEMICAL SCIENCE
 |
| 出版日期 | 2016 |
| 卷号 | 7期号:3页码:1891-1895 |
| DOI | 10.1039/c5sc03404c |
| 文献子类 | Article |
| 英文摘要 | We report the chemical synthesis of the first photo-activatable protein antigen that can be used to study antigen-antibody interaction mediated responses in B cells. This strategy facilitated fine tuning of the caged protein antigen to optimize its bioactivity and photochemical properties. One optimal molecule, HEL-K96NPE, was totally inert to hen egg lysozyme (HEL)-specific B cells and could only restore its antigenicity upon photoactivation. Combined with real time live cell imaging, the utility of HEL-K96NPE was demonstrated as a proof of concept to quantify B cell synapse formation and calcium influx responses at the single cell level. |
| WOS关键词 | INTERNAL-REFLECTION FLUORESCENCE ; B-CELL ACTIVATION ; MEDIATED INTERACTIONS ; PEPTIDE HYDRAZIDES ; CRYSTAL-STRUCTURE ; PROTECTING GROUPS ; FAB COMPLEX ; IN-VIVO ; LYSOZYME ; RECEPTOR |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000371021900030 |
| 资助机构 | MOST(2014CB542500 ; MOST(2014CB542500 ; MOST(2014CB542500 ; MOST(2014CB542500 ; NSFC(81361120384 ; NSFC(81361120384 ; NSFC(81361120384 ; NSFC(81361120384 ; Specialized Research Fund for the Doctoral Program of Higher Education(20120002130004) ; Specialized Research Fund for the Doctoral Program of Higher Education(20120002130004) ; Specialized Research Fund for the Doctoral Program of Higher Education(20120002130004) ; Specialized Research Fund for the Doctoral Program of Higher Education(20120002130004) ; 2013CB932800 ; 2013CB932800 ; 2013CB932800 ; 2013CB932800 ; 21532004 ; 21532004 ; 21532004 ; 21532004 ; 2014AA020527) ; 2014AA020527) ; 2014AA020527) ; 2014AA020527) ; 31270913 ; 31270913 ; 31270913 ; 31270913 ; 81422020) ; 81422020) ; 81422020) ; 81422020) ; MOST(2014CB542500 ; MOST(2014CB542500 ; MOST(2014CB542500 ; MOST(2014CB542500 ; NSFC(81361120384 ; NSFC(81361120384 ; NSFC(81361120384 ; NSFC(81361120384 ; Specialized Research Fund for the Doctoral Program of Higher Education(20120002130004) ; Specialized Research Fund for the Doctoral Program of Higher Education(20120002130004) ; Specialized Research Fund for the Doctoral Program of Higher Education(20120002130004) ; Specialized Research Fund for the Doctoral Program of Higher Education(20120002130004) ; 2013CB932800 ; 2013CB932800 ; 2013CB932800 ; 2013CB932800 ; 21532004 ; 21532004 ; 21532004 ; 21532004 ; 2014AA020527) ; 2014AA020527) ; 2014AA020527) ; 2014AA020527) ; 31270913 ; 31270913 ; 31270913 ; 31270913 ; 81422020) ; 81422020) ; 81422020) ; 81422020) |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/22313]  |
| 专题 | 合肥物质科学研究院_中科院强磁场科学中心 |
| 作者单位 | 1.Tsinghua Univ, Dept Chem, Tsinghua Peking Ctr Life Sci, Key Lab Bioorgan Phosphorus Chem & Chem Biol,Mini, Beijing 100084, Peoples R China 2.Tsinghua Univ, Sch Life Sci, Collaborat Innovat Ctr Diag & Treatment Infect Di, MOE Key Lab Prot Sci, Beijing 100084, Peoples R China 3.Chinese Acad Sci, High Magnet Field Lab, Hefei 230031, Peoples R China 4.Tsinghua Univ, Sch Med, Lab Dynam Immunobiol, Beijing 100084, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tang, Shan,Wan, Zhengpeng,Gao, Yiren,et al. Total chemical synthesis of photoactivatable proteins for light-controlled manipulation of antigen-antibody interactions[J]. CHEMICAL SCIENCE,2016,7(3):1891-1895. |
| APA | Tang, Shan.,Wan, Zhengpeng.,Gao, Yiren.,Zheng, Ji-Shen.,Wang, Jing.,...&Liu, Wanli.(2016).Total chemical synthesis of photoactivatable proteins for light-controlled manipulation of antigen-antibody interactions.CHEMICAL SCIENCE,7(3),1891-1895. |
| MLA | Tang, Shan,et al."Total chemical synthesis of photoactivatable proteins for light-controlled manipulation of antigen-antibody interactions".CHEMICAL SCIENCE 7.3(2016):1891-1895. |
入库方式： OAI收割
来源：合肥物质科学研究院
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