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  4. 中科院强磁场科学中心
Using "On/Off" F-19 NMR/Magnetic Resonance Imaging Signals to Sense Tyrosine Kinase/Phosphatase Activity in Vitro and in Cell Lysates

文献类型：期刊论文

作者Zheng, Zhen1; Sun, Hongbin2; Hu, Chen2; Li, Gongyu1; Liu, Xiaomei1; Chen, Peiyao1; Cui, Yusi1; Liu, Jing2; Wang, Junfeng2; Liang, Gaolin1
刊名ANALYTICAL CHEMISTRY
出版日期2016-03-15
卷号88期号:6页码:3363-3368
DOI10.1021/acs.analchem.6b00036
文献子类Article
英文摘要Tyrosine kinase and phosphatase are two important, antagonistic enzymes in organisms. Development of noninvasive approach for sensing their activity with high spatial and temporal resolution remains challenging. Herein, we rationally designed a hydrogelator Nap-Phe-Phe(CF3)-Glu-Tyr-Ile-OH (1a) whose supramolecular hydrogel (i.e., Gel 1a) can be subjected to tyrosine kinase-directed disassembly, and its phosphate precursor Nap-Phe-Phe(CF3)-Glu-Tyr(H2PO3)-Ile-OH (1b) which: can be subjected to alkaline phosphatase (ALP)-instructed self-assembly to fonn supramolecular hydro gel Gel 1b, respectively.. Mechanic properties and internal fibrous networks of the hydrogels were characterized with rheology and cryo transmission electron microscopy (cryo-TEM). Disassembly/Self-asSemblY of their corresponding supramolecular hydrogels conferring respective "On/Off' F-19 NMR/MRI signals were employed to sense the activity of these two important enzymes in vitro and in cell lysates for the first time. We anticipate that our new F-19 NMR/magnetic, resonance imaging (MRI) method would facilitate pharmaceutical researchers to screen new inhibitors for these two enzymes without steric hindrance.
WOS关键词PROTEIN-KINASE ACTIVITY ; ENZYMATIC HYDROGELATION ; PHOSPHATASE-ACTIVITIES ; NANOPARTICLES ; PHOSPHORYLATION ; NANOPROBES ; PROBES ; SWITCH ; VIVO ; MRI
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000372391500053
资助机构Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; National Natural Science Foundation of China(U1332142 ; National Natural Science Foundation of China(U1332142 ; National Natural Science Foundation of China(U1332142 ; National Natural Science Foundation of China(U1332142 ; National Natural Science Foundation of China(U1332142 ; National Natural Science Foundation of China(U1332142 ; National Natural Science Foundation of China(U1332142 ; National Natural Science Foundation of China(U1332142 ; 21375121) ; 21375121) ; 21375121) ; 21375121) ; 21375121) ; 21375121) ; 21375121) ; 21375121) ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; National Natural Science Foundation of China(U1332142 ; National Natural Science Foundation of China(U1332142 ; National Natural Science Foundation of China(U1332142 ; National Natural Science Foundation of China(U1332142 ; National Natural Science Foundation of China(U1332142 ; National Natural Science Foundation of China(U1332142 ; National Natural Science Foundation of China(U1332142 ; National Natural Science Foundation of China(U1332142 ; 21375121) ; 21375121) ; 21375121) ; 21375121) ; 21375121) ; 21375121) ; 21375121) ; 21375121)
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/22340]  
专题合肥物质科学研究院_中科院强磁场科学中心
作者单位1.Univ Sci & Technol China, Dept Chem, Hefei Sci Ctr CAS, CAS Key Lab Soft Matter Chem, Hefei 230026, Anhui, Peoples R China
2.Chinese Acad Sci, Hefei Inst Phys Sci, High Field Magnet Lab, Hefei 230031, Anhui, Peoples R China
推荐引用方式
GB/T 7714		 Zheng, Zhen,Sun, Hongbin,Hu, Chen,et al. Using "On/Off" F-19 NMR/Magnetic Resonance Imaging Signals to Sense Tyrosine Kinase/Phosphatase Activity in Vitro and in Cell Lysates[J]. ANALYTICAL CHEMISTRY,2016,88(6):3363-3368.
APA Zheng, Zhen.,Sun, Hongbin.,Hu, Chen.,Li, Gongyu.,Liu, Xiaomei.,...&Liang, Gaolin.(2016).Using "On/Off" F-19 NMR/Magnetic Resonance Imaging Signals to Sense Tyrosine Kinase/Phosphatase Activity in Vitro and in Cell Lysates.ANALYTICAL CHEMISTRY,88(6),3363-3368.
MLA Zheng, Zhen,et al."Using "On/Off" F-19 NMR/Magnetic Resonance Imaging Signals to Sense Tyrosine Kinase/Phosphatase Activity in Vitro and in Cell Lysates".ANALYTICAL CHEMISTRY 88.6(2016):3363-3368.

入库方式： OAI收割

来源：合肥物质科学研究院

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