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  2. 合肥物质科学研究院
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  4. 中科院强磁场科学中心
Combined approaches of EPR and NMR illustrate only one transmembrane helix in the human IFITM3

文献类型：期刊论文

作者Ling, Shenglong1; Zhang, Chengwei1; Wang, Wei1; Cai, Xiaoying1; Yu, Lu2; Wu, Fangming2; Zhang, Longhua1; Tian, Changlin1,2
刊名SCIENTIFIC REPORTS
出版日期2016-04-05
卷号6期号:无页码:1-8
DOI10.1038/srep24029
文献子类Article
英文摘要Interferon-inducible transmembrane protein IFITM3 was known to restrict the entry of a wide spectrum of viruses to the cytosol of the host. The mechanism used by the protein to restrict viral entry is unclear given the unavailability of the membrane topology and structures of the IFITM family proteins. Systematic site-directed spin labeling (SDSL) and electron paramagnetic resonance (EPR) studies of IFITM3 in detergent micelles identified a single, long transmembrane helix in the C-terminus and an intramembrane segment in the N-terminal hydrophobic region. Solution NMR studies of the same sample verified the secondary structure distribution and demonstrated two rigid regions interacting with the micellar surface. The resulting membrane topology of IFITM3 supports the mechanism of an enhanced restricted membrane hemi-fusion.
WOS关键词ANTIVIRAL ACTIVITY ; S-PALMITOYLATION ; FAMILY PROTEINS ; DENGUE VIRUS ; INTERFERON ; DOMAIN ; CELLS ; REPLICATION ; MECHANISMS ; RESISTANCE
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000373339000001
资助机构National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; 21473176 ; 21473176 ; 21473176 ; 21473176 ; 31470740) ; 31470740) ; 31470740) ; 31470740) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Key Science Research Grant of Ministry of Science and Technology, China(2015CB910100) ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; National Natural Science Foundation of China(U1332138 ; 21473176 ; 21473176 ; 21473176 ; 21473176 ; 31470740) ; 31470740) ; 31470740) ; 31470740)
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/22342]  
专题合肥物质科学研究院_中科院强磁场科学中心
作者单位1.Univ Sci & Technol China, Sch Life Sci, Hefei Natl Lab Microscale Phys Sci, Hefei 230027, Peoples R China
2.Chinese Acad Sci, High Magnet Field Lab, Hefei 230031, Peoples R China
推荐引用方式
GB/T 7714		 Ling, Shenglong,Zhang, Chengwei,Wang, Wei,et al. Combined approaches of EPR and NMR illustrate only one transmembrane helix in the human IFITM3[J]. SCIENTIFIC REPORTS,2016,6(无):1-8.
APA Ling, Shenglong.,Zhang, Chengwei.,Wang, Wei.,Cai, Xiaoying.,Yu, Lu.,...&Tian, Changlin.(2016).Combined approaches of EPR and NMR illustrate only one transmembrane helix in the human IFITM3.SCIENTIFIC REPORTS,6(无),1-8.
MLA Ling, Shenglong,et al."Combined approaches of EPR and NMR illustrate only one transmembrane helix in the human IFITM3".SCIENTIFIC REPORTS 6.无(2016):1-8.

入库方式： OAI收割

来源：合肥物质科学研究院

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