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Autophagic flux is highly active in early mitosis and differentially regulated throughout the cell cycle

文献类型：期刊论文

作者Li, Zhiyuan1; Ji, Xinmiao1; Wang, Dongmei1,2; Liu, Juanjuan1,2; Zhang, Xin1
刊名ONCOTARGET
出版日期2016-06-28
卷号7期号:26页码:39705-39718
关键词Mitosis Autophagy Autophagic Flux Lc3 Cell Synchronization
DOI10.18632/oncotarget.9451
文献子类Article
英文摘要Mitosis is a fast process that involves dramatic cellular remodeling and has a high energy demand. Whether autophagy is active or inactive during the early stages of mitosis in a naturally dividing cell is still debated. Here we aimed to use multiple assays to resolve this apparent discrepancy. Although the LC3 puncta number was reduced in mitosis, the four different cell lines we tested all have active autophagic flux in both interphase and mitosis. In addition, the autophagic flux was highly active in nocodazole-induced, double-thymidine synchronization released as well as naturally occurring mitosis in HeLa cells. Multiple autophagy proteins are upregulated in mitosis and the increased Beclin-1 level likely contributes to the active autophagic flux in early mitosis. It is interesting that although the autophagic flux is active throughout the cell cycle, early mitosis and S phase have relatively higher autophagic flux than G1 and late G2 phases, which might be helpful to degrade the damaged organelles and provide energy during S phase and mitosis.
WOS关键词CLATHRIN-MEDIATED ENDOCYTOSIS ; ANIMAL-CELLS ; CYTOKINESIS ; INHIBITION ; AMMONIA ; BIOGENESIS ; MITOPHAGY ; DYNAMICS ; PERSISTS ; VPS34
WOS研究方向Oncology ; Cell Biology
语种英语
WOS记录号WOS:000378614700058
资助机构Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; National Natural Science Foundation of China(31301112) ; National Natural Science Foundation of China(31301112) ; National Natural Science Foundation of China(31301112) ; National Natural Science Foundation of China(31301112) ; National Natural Science Foundation of China(31301112) ; National Natural Science Foundation of China(31301112) ; National Natural Science Foundation of China(31301112) ; National Natural Science Foundation of China(31301112) ; Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; National Natural Science Foundation of China(31301112) ; National Natural Science Foundation of China(31301112) ; National Natural Science Foundation of China(31301112) ; National Natural Science Foundation of China(31301112) ; National Natural Science Foundation of China(31301112) ; National Natural Science Foundation of China(31301112) ; National Natural Science Foundation of China(31301112) ; National Natural Science Foundation of China(31301112)
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/22463]  
专题合肥物质科学研究院_中科院强磁场科学中心
作者单位1.Chinese Acad Sci, High Field Magnet Lab, Hefei 230031, Anhui, Peoples R China
2.Univ Sci & Technol China, Hefei 230036, Anhui, Peoples R China
推荐引用方式
GB/T 7714		 Li, Zhiyuan,Ji, Xinmiao,Wang, Dongmei,et al. Autophagic flux is highly active in early mitosis and differentially regulated throughout the cell cycle[J]. ONCOTARGET,2016,7(26):39705-39718.
APA Li, Zhiyuan,Ji, Xinmiao,Wang, Dongmei,Liu, Juanjuan,&Zhang, Xin.(2016).Autophagic flux is highly active in early mitosis and differentially regulated throughout the cell cycle.ONCOTARGET,7(26),39705-39718.
MLA Li, Zhiyuan,et al."Autophagic flux is highly active in early mitosis and differentially regulated throughout the cell cycle".ONCOTARGET 7.26(2016):39705-39718.

入库方式： OAI收割

来源：合肥物质科学研究院

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