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Reinforcement of integrin-mediated T-Lymphocyte adhesion by TNF-induced Inside-out Signaling

文献类型：期刊论文

作者Li, Qian1,3; Huth, Steven1; Adam, Dieter2; Selhuber-Unkel, Christine1
刊名Scientific Reports
出版日期2016-07-28
卷号6期号:无页码:1-10
DOI10.1038/srep30452
文献子类Article
英文摘要Integrin-mediated leukocyte adhesion to endothelial cells is a crucial step in immunity against pathogens. Whereas the outside-in signaling pathway in response to the pro-inflammatory cytokine tumour necrosis factor (TNF) has already been studied in detail, little knowledge exists about a supposed TNF-mediated inside-out signaling pathway. In contrast to the outside-in signaling pathway, which relies on the TNF-induced upregulation of surface molecules on endothelium, inside-out signaling should also be present in an endothelium-free environment. Using single-cell force spectroscopy, we show here that stimulating Jurkat cells with TNF significantly reinforces their adhesion to fibronectin in a biomimetic in vitro assay for cell-surface contact times of about 1.5 seconds, whereas for larger contact times the effect disappears. Analysis of single-molecule ruptures further demonstrates that TNF strengthens sub-cellular single rupture events at short cell-surface contact times. Hence, our results provide quantitative evidence for the significant impact of TNF-induced inside-out signaling in the T-lymphocyte initial adhesion machinery.
WOS关键词CELL FORCE SPECTROSCOPY ; WD-REPEAT PROTEIN ; NEUTRAL SPHINGOMYELINASE ; EXTRACELLULAR-MATRIX ; RECEPTOR ; FIBRONECTIN ; BONDS ; ACTIVATION ; MODULATION ; MOLECULE-1
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000380726600001
资助机构Deutsche Forschungsgemeinschaft(SFB 877 ; Deutsche Forschungsgemeinschaft(SFB 877 ; Deutsche Forschungsgemeinschaft(SFB 877 ; Deutsche Forschungsgemeinschaft(SFB 877 ; Deutsche Forschungsgemeinschaft(SFB 877 ; Deutsche Forschungsgemeinschaft(SFB 877 ; Deutsche Forschungsgemeinschaft(SFB 877 ; Deutsche Forschungsgemeinschaft(SFB 877 ; ERC(336104) ; ERC(336104) ; ERC(336104) ; ERC(336104) ; ERC(336104) ; ERC(336104) ; ERC(336104) ; ERC(336104) ; EXC306-PMTP1 ; EXC306-PMTP1 ; EXC306-PMTP1 ; EXC306-PMTP1 ; EXC306-PMTP1 ; EXC306-PMTP1 ; EXC306-PMTP1 ; EXC306-PMTP1 ; EXC306-PWTP2 ; EXC306-PWTP2 ; EXC306-PWTP2 ; EXC306-PWTP2 ; EXC306-PWTP2 ; EXC306-PWTP2 ; EXC306-PWTP2 ; EXC306-PWTP2 ; SFB 677 ; SFB 677 ; SFB 677 ; SFB 677 ; SFB 677 ; SFB 677 ; SFB 677 ; SFB 677 ; SE 1801/2-1 ; SE 1801/2-1 ; SE 1801/2-1 ; SE 1801/2-1 ; SE 1801/2-1 ; SE 1801/2-1 ; SE 1801/2-1 ; SE 1801/2-1 ; INST 257/407-1) ; INST 257/407-1) ; INST 257/407-1) ; INST 257/407-1) ; INST 257/407-1) ; INST 257/407-1) ; INST 257/407-1) ; INST 257/407-1) ; Deutsche Forschungsgemeinschaft(SFB 877 ; Deutsche Forschungsgemeinschaft(SFB 877 ; Deutsche Forschungsgemeinschaft(SFB 877 ; Deutsche Forschungsgemeinschaft(SFB 877 ; Deutsche Forschungsgemeinschaft(SFB 877 ; Deutsche Forschungsgemeinschaft(SFB 877 ; Deutsche Forschungsgemeinschaft(SFB 877 ; Deutsche Forschungsgemeinschaft(SFB 877 ; ERC(336104) ; ERC(336104) ; ERC(336104) ; ERC(336104) ; ERC(336104) ; ERC(336104) ; ERC(336104) ; ERC(336104) ; EXC306-PMTP1 ; EXC306-PMTP1 ; EXC306-PMTP1 ; EXC306-PMTP1 ; EXC306-PMTP1 ; EXC306-PMTP1 ; EXC306-PMTP1 ; EXC306-PMTP1 ; EXC306-PWTP2 ; EXC306-PWTP2 ; EXC306-PWTP2 ; EXC306-PWTP2 ; EXC306-PWTP2 ; EXC306-PWTP2 ; EXC306-PWTP2 ; EXC306-PWTP2 ; SFB 677 ; SFB 677 ; SFB 677 ; SFB 677 ; SFB 677 ; SFB 677 ; SFB 677 ; SFB 677 ; SE 1801/2-1 ; SE 1801/2-1 ; SE 1801/2-1 ; SE 1801/2-1 ; SE 1801/2-1 ; SE 1801/2-1 ; SE 1801/2-1 ; SE 1801/2-1 ; INST 257/407-1) ; INST 257/407-1) ; INST 257/407-1) ; INST 257/407-1) ; INST 257/407-1) ; INST 257/407-1) ; INST 257/407-1) ; INST 257/407-1)
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/22498]  
专题合肥物质科学研究院_中科院强磁场科学中心
作者单位1.Univ Kiel, Biocompatible Nanomat, Inst Mat Sci, Kaiserstr 2, D-24143 Kiel, Germany
2.Univ Kiel, Inst Immunol, Michaelisstr 5, D-24105 Kiel, Germany
3.Chinese Acad Sci, High Field Magnet Lab, Hefei 230031, Anhui, Peoples R China
推荐引用方式
GB/T 7714		 Li, Qian,Huth, Steven,Adam, Dieter,et al. Reinforcement of integrin-mediated T-Lymphocyte adhesion by TNF-induced Inside-out Signaling[J]. Scientific Reports,2016,6(无):1-10.
APA Li, Qian,Huth, Steven,Adam, Dieter,&Selhuber-Unkel, Christine.(2016).Reinforcement of integrin-mediated T-Lymphocyte adhesion by TNF-induced Inside-out Signaling.Scientific Reports,6(无),1-10.
MLA Li, Qian,et al."Reinforcement of integrin-mediated T-Lymphocyte adhesion by TNF-induced Inside-out Signaling".Scientific Reports 6.无(2016):1-10.

入库方式： OAI收割

来源：合肥物质科学研究院

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