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Fluorogenic Enhancement of an in Vitro-Selected Peptide Ligand by Replacement of a Fluorescent Group

文献类型：期刊论文

作者Wang, Wei1,2; Zhu, Liping2; Hirano, Yoshinori3; Karirninavargani, Marziyeh2,4; Tada, Seiichi6; Zhang, Guanxin5; Uzawa, Takanori2,6; Zhang, Deqing5; Hirose, Takuji4; Taiji, Makoto3
刊名ANALYTICAL CHEMISTRY
出版日期2016-08-16
卷号88期号:16页码:7991-7997
DOI10.1021/acs.analchem.6b01032
文献子类Article
英文摘要To prepare a fluorogenic peptide ligand which 'binds to an arbitrary target, we previously succeeded in seeking a fluorogenic ligand to calmodulin using in vitro selection. In this study the environment-sensitive fluorescent group in the selected peptide ligand was replaced with other fluorescent groups to ad, the possibility-to increase the fluorogenic activity. Surface plasmon resonance measurement exhibited that the binding affinity was held even after the replacement. However, the replacement significantly affected the fluorogenic activity. It depended on the kind of incorporated fluorophors and linker length. As a result, the incorporation of 4-N,N-dimethylamino-1,8-naphthalimide enhanced the fluorescence intensity over 100-fold' in the presence of target calcium-bound calModulin. This study demonstrated that the functionality of in vitro selected peptide can, be tuned with keeping the binding affinity.
WOS关键词AGGREGATION-INDUCED EMISSION ; DYNAMIC PROTEIN INTERACTIONS ; AMINO-ACID ; APTAMER ; PROBES ; IMMUNOASSAY ; POSITION ; ANALOG ; CELLS ; ASSAY
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000381654800018
资助机构JSPS(22220009 ; JSPS(22220009 ; JSPS(22220009 ; JSPS(22220009 ; JSPS(22220009 ; JSPS(22220009 ; JSPS(22220009 ; JSPS(22220009 ; 15H01810) ; 15H01810) ; 15H01810) ; 15H01810) ; 15H01810) ; 15H01810) ; 15H01810) ; 15H01810) ; JSPS(22220009 ; JSPS(22220009 ; JSPS(22220009 ; JSPS(22220009 ; JSPS(22220009 ; JSPS(22220009 ; JSPS(22220009 ; JSPS(22220009 ; 15H01810) ; 15H01810) ; 15H01810) ; 15H01810) ; 15H01810) ; 15H01810) ; 15H01810) ; 15H01810)
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/24394]  
专题合肥物质科学研究院_中科院强磁场科学中心
作者单位1.Chinese Acad Sci, High Field Magnet Lab, Hefei 230031, Anhui, Peoples R China
2.RIKEN, Nano Med Engn Lab, 2-1 Hirosawa, Wako, Saitama 3510198, Japan
3.RIKEN, Quantitat Biol Ctr, Computat Biol Res Core, Lab Computat Mol Design, 2F,QBiC Bldg B,6-2-4 Furuedai, Suita, Osaka 5650874, Japan
4.Saitama Univ, Grad Sch Sci & Engn, Sakura Ku, 255 Shimo Okubo, Saitama 3388570, Japan
5.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Organ Solids, Beijing 100190, Peoples R China
6.RIKEN, Ctr Emergent Matter Sci, Emergent Bioengn Mat Res Team, 2-1 Hirosawa, Wako, Saitama 3510198, Japan
推荐引用方式
GB/T 7714		 Wang, Wei,Zhu, Liping,Hirano, Yoshinori,et al. Fluorogenic Enhancement of an in Vitro-Selected Peptide Ligand by Replacement of a Fluorescent Group[J]. ANALYTICAL CHEMISTRY,2016,88(16):7991-7997.
APA Wang, Wei.,Zhu, Liping.,Hirano, Yoshinori.,Karirninavargani, Marziyeh.,Tada, Seiichi.,...&Ito, Yoshihiro.(2016).Fluorogenic Enhancement of an in Vitro-Selected Peptide Ligand by Replacement of a Fluorescent Group.ANALYTICAL CHEMISTRY,88(16),7991-7997.
MLA Wang, Wei,et al."Fluorogenic Enhancement of an in Vitro-Selected Peptide Ligand by Replacement of a Fluorescent Group".ANALYTICAL CHEMISTRY 88.16(2016):7991-7997.

入库方式： OAI收割

来源：合肥物质科学研究院

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