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  4. 中科院合肥智能机械研究所
Real-Time Discrimination and Versatile Profiling of Spontaneous Reactive Oxygen Species in Living Organisms with a Single Fluorescent Probe

文献类型：期刊论文

作者Zhang, Ruilong1,2; Zhao, Jun1; Han, Guangmei1; Liu, Zhengjie1,2; Liu, Cui1,2; Zhang, Cheng1,2; Liu, Bianhua1; Jiang, Changlong1; Liu, Renyong1; Zhao, Tingting1
刊名JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
出版日期2016-03-23
卷号138期号:11页码:3769-3778
DOI10.1021/jacs.5b12848
文献子类Article
英文摘要Fluorescent probes are powerful tools for the investigations of reactive oxygen species (ROS) in living organisms by visualization and imaging. However, the multiparallel assays of several ROS with multiple probes are often limited by the available number of spectrally nonoverlapping chromophores together with large invasive effects and discrepant biological locations. Meanwhile, the spontaneous ROS profilings in various living organs/tissues are also limited by the penetration capability of probes across different biological barriers and the stability in reactive in vivo environments. Here, we report a single fluorescent probe to achieve the effective discrimination and profiling of hydroxyl radicals (center dot OH) and hypochlorous acid (HClO) in living organisms. The probe is constructed by chemically grafting an additional five-membered heterocyclic ring and a lateral triethylene glycol chain to a fluorescein mother, which does not only turn off the fluorescence of fluorescein, but also create the dual reactive sites to ROS and the penetration capability in passing through various biological barriers. The reactions of probe with center dot OH and HClO simultaneously result in cyan and green emissions, respectively, providing the real-time discrimination and quantitative analysis of the two ROS in cellular mitochondria. Surprisingly, the accumulation of probes in the intestine and liver of a normal-state zebrafish and the transfer pathway from intestine-to-blood-to-organ/tissue-to-kidney-to-excretion clearly present the profiling of spontaneous center dot OH and HClO in these metabolic organs. In particular, the stress generation of center dot OH at the fresh wound of zebrafish is successfully visualized for the first time, in spite of its extremely short lifetime.
WOS关键词HYPOCHLOROUS ACID DETECTION ; HYDROGEN-PEROXIDE ; IN-VIVO ; OXIDATIVE STRESS ; FREE-RADICALS ; CELLS ; SUPEROXIDE ; MECHANISMS ; OXIDANTS ; DISEASE
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000372854200025
资助机构National Basic Research Program of China(2015CB932002) ; National Basic Research Program of China(2015CB932002) ; National Basic Research Program of China(2015CB932002) ; National Basic Research Program of China(2015CB932002) ; China-Singapore Joint Project(2015DFG92510) ; China-Singapore Joint Project(2015DFG92510) ; China-Singapore Joint Project(2015DFG92510) ; China-Singapore Joint Project(2015DFG92510) ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; Natural Science Foundation of Anhui Province(1408085MKL52 ; Natural Science Foundation of Anhui Province(1408085MKL52 ; Natural Science Foundation of Anhui Province(1408085MKL52 ; Natural Science Foundation of Anhui Province(1408085MKL52 ; 21475135 ; 21475135 ; 21475135 ; 21475135 ; 1508085SQB200 ; 1508085SQB200 ; 1508085SQB200 ; 1508085SQB200 ; 21375131 ; 21375131 ; 21375131 ; 21375131 ; 1608085QB32) ; 1608085QB32) ; 1608085QB32) ; 1608085QB32) ; 21275145 ; 21275145 ; 21275145 ; 21275145 ; 21371174) ; 21371174) ; 21371174) ; 21371174) ; National Basic Research Program of China(2015CB932002) ; National Basic Research Program of China(2015CB932002) ; National Basic Research Program of China(2015CB932002) ; National Basic Research Program of China(2015CB932002) ; China-Singapore Joint Project(2015DFG92510) ; China-Singapore Joint Project(2015DFG92510) ; China-Singapore Joint Project(2015DFG92510) ; China-Singapore Joint Project(2015DFG92510) ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; Natural Science Foundation of Anhui Province(1408085MKL52 ; Natural Science Foundation of Anhui Province(1408085MKL52 ; Natural Science Foundation of Anhui Province(1408085MKL52 ; Natural Science Foundation of Anhui Province(1408085MKL52 ; 21475135 ; 21475135 ; 21475135 ; 21475135 ; 1508085SQB200 ; 1508085SQB200 ; 1508085SQB200 ; 1508085SQB200 ; 21375131 ; 21375131 ; 21375131 ; 21375131 ; 1608085QB32) ; 1608085QB32) ; 1608085QB32) ; 1608085QB32) ; 21275145 ; 21275145 ; 21275145 ; 21275145 ; 21371174) ; 21371174) ; 21371174) ; 21371174)
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/30903]  
专题合肥物质科学研究院_中科院合肥智能机械研究所
作者单位1.Chinese Acad Sci, Ctr Excellence Nanosci, Inst Intelligent Machines, Hefei 230031, Anhui, Peoples R China
2.Univ Sci & Technol China, Dept Chem, Hefei 230026, Anhui, Peoples R China
3.ASTAR, Inst Mat Res & Engn, 3 Res Link, Singapore 117602, Singapore
4.Chinese Acad Sci, State Key Lab Transducer Technol, Hefei 230031, Anhui, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Ruilong,Zhao, Jun,Han, Guangmei,et al. Real-Time Discrimination and Versatile Profiling of Spontaneous Reactive Oxygen Species in Living Organisms with a Single Fluorescent Probe[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2016,138(11):3769-3778.
APA Zhang, Ruilong.,Zhao, Jun.,Han, Guangmei.,Liu, Zhengjie.,Liu, Cui.,...&Zhang, Zhongping.(2016).Real-Time Discrimination and Versatile Profiling of Spontaneous Reactive Oxygen Species in Living Organisms with a Single Fluorescent Probe.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,138(11),3769-3778.
MLA Zhang, Ruilong,et al."Real-Time Discrimination and Versatile Profiling of Spontaneous Reactive Oxygen Species in Living Organisms with a Single Fluorescent Probe".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 138.11(2016):3769-3778.

入库方式： OAI收割

来源：合肥物质科学研究院

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